Summary
Examples are given of observations made with field isolates of suid herpesvirus 1 (SHV-1) which indicate that intergenomic recombination is a common phenomenon associated with the virus. This was further confirmed by experimental co-infection of a pig with 2 virus strains with different, stable and easily identifiable genomic markers, followed by natural transmission to a group of contact pigs. A variety of recombinants was subsequently isolated, while none of the parental strains were re-isolated from any of the pigs. It is suggested that co-invasion of cells and recombination between viral genomes play a role in the life cycle of the virus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ben-Porat T, Deatly A, Veach RA, Blankenship ML (1984) Equalization of the inverted repeat sequences of the pseudorabies virus genome by intermolecular recombination. Virology 132: 303–314
Ben-Porat T, Kaplan AS (1984) Molecular biology of pseudorabies virus. In: Roizman B (ed) The herpesviruses, vol 3. Plenum Press, New York, pp 105–173
Ben-Porat T, Wu C, Harper L, Lomniczi B (1984) Biological significance of the alterations in restriction patterns of the genomes of different isolates of pseudorabies virus. In: Rapp F (ed) Herpesvirus. UCLA Symposia of Molecular and Cellular Biology, New Series 21. Alan R. Liss Inc., New York, pp 537–550
Chowdhury SI, Buhk H-J, Ludwig H, Hammerschmidt W (1990) Genomic termini of Equine herpesvirus 1. J Virol 64: 873–880
Christensen LS (1988) Comparison by restriction fragment pattern analyses and molecular characterization of some European isolates of suid herpesvirus 1: a contribution to strain differentiation of European isolates. Arch Virol 102: 39–47
Christensen LS, Medveczky I, Strandbygaard BS, Pejsak Z (1992) Characterization of field isolates of suid herpesvirus 1 (Aujeszky's disease virus) as derivatives of attenuated vaccine strains. Arch Virol 124: 225–234
Christensen LS, Mortensen S, Boetner A, Strandbygaard BS, Roensholdt L, Henriksen CA, Andersen JB (1993) Further evidence of long distance airborne transmission of Aujeszky's disease (pseudorabies) virus. Vet Rec 132: 317–321
Christensen LS, Mousing J, Mortensen S, Sørensen, Strandbygaard SB, Henriksen CA, Andersen JB (1990) Evidence of long distance airborne transmission of Aujeszky's disease (pseudorabies) virus. Vet Rec 127: 471–474
Christensen LS, Normann P (1992) A simple method for purification of herpesvirus DNA. J Virol Methods 37: 99–102
Christensen LS, Sørensen KJ (1991) The genomic diversity and stability of field strains of suid herpesvirus 1 (Aujeszky's disease virus). Vet Microbiol 26: 1–10
Christensen LS, Sørensen KJ, Lei JC (1987) Restriction fragment pattern (RFP) analysis of genomes from Danish isolates of Suid herpesvirus 1 (Aujeszky's disease virus). Arch Virol 97: 215–224
Espuna E, Kovacs F, Hejja I, Riere P, Lomniczi B (1990) Variantes genéticas del virus de la enfermeded de Aujeszky en Espana. Med Vet 7: 27–33
Gielkens AJL, Van Oirschot JT, Berns AJM (1985) Genome differences among field isolates and vaccine strains of pseudorabies virus. J Gen Virol 66: 69–82
Hammerschmidt W, Ludwig H, Buhk H-J (1986) Short repeats cause heterogeneity at genomic terminus of bovine herpesvirus 1. J Virol 58: 43–49
Hammerschmidt W, Ludwig H, Buhk H-J (1988) Specificity of cleavage in replicative-form DNA of bovine herpesvirus 1. J Virol 62: 1355–1363
Henderson LM, Levings RL, Davis AJ, Sturtz DR (1991) Recombination of pseudorabies virus vaccine strains in swine. Am J Vet Res 52: 820–825
Herrmann SC, Heppner B, Ludwig H (1984) Pseudorabies virus from clinical outbreaks and latent infections grouped into four major genome types. In: Wittmann G, Gaskell RM, Rziha H-J (eds) Latent herpesvirus infections in veterinary medicine. M. Nijhoff, Boston, pp 387–401 (Current topics in veterinary medicine and animal sciences, vol 27)
Iglesias JG, Harkness JW (1988) Studies of transplacental and perinatal infection with two clones if a single Aujeszky's disease (pseudorabies) virus isolate. Vet Microbiol 16: 243–254
Kit S (1989) Safety and efficacy of genetically engineered Aujeszky's disease vaccines. In: Van Oirschot JT (ed) Vaccination and control of Aujeszky's disease. Kluver Academic Publisher, Dordrecht, pp 45–56 (Current topics in veterinary medicine and animal sciences, vol 49)
Lomniczi B, Nagy E, Kükedi A, Zsák L (1989) Molecular epidemiology of Aujeszky's disease in Hungary. In: van Oirschot JT (ed) Vaccination and control Aujeszky's disease. Kluver Academic Publisher, Dordrecht, pp 93–102 (Current topics in veterinary medicine and animal sciences, vol 49)
Mengeling WL, Paul PS, Pirtle EC, Wathen MW (1983) Restriction endonuclease analysis of pseudorabies (Aujeszky's disease) virus before and after serial passage in vivo and in vitro. Arch Virol 78: 213–220
Paul PS, Mengeling WL, Pirtle EC (1982) Differentiation of pseudorabies (Aujeszky's disease) virus strains by restriction endonuclease analysis. Arch Virol 73: 193–198
Simon A, Mettenleiter TC, Rziha H-J (1989) Pseudorabies virus displays variable numbers of a repeat unit adjacent to the 3′ end of the glycoprotein gII gene. J Gen Virol 70: 1239–1246
Wathen MW, Pirtle EC (1984) Stability of the pseudorabies virus genome after in vivo serial passage. J Gen Virol 65: 1401–1404
Zhang G, Leader DP (1990) The structure of the pseudorabies virus genome at the end of the inverted repeat sequences proximal to the junction with the short unique region. J Gen Virol 71: 2433–2441
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Christensen, L.S., Lomniczi, B. High frequency intergenomic recombination of suid herpesvirus 1 (SHV-1, Aujeszky's disease virus). Archives of Virology 132, 37–50 (1993). https://doi.org/10.1007/BF01309842
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309842