Summary
Vero cell cultures persistently infected with Junin virus and subjected to different cultural conditions were established. The production of infectious plaque-forming virus,ts mutants and interfering viral particles was determined at different times during 110 days after infection. Carrier cultures maintained in stationary conditions continuously released PFU while proliferating persistent cultures exhibited a cyclical pattern which tends to a rapid PFU disappearance. Concomitantly, in stationary cultures the production of interfering particles was delayed and was lower than in actively growing persistent cells. The metabolic state of the infected cells did not affect the release ofts mutants. The results suggest that a cellular function is involved on the regulation of Junin virus persistent infections.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bedows, E., Payne, F. E.: Host cell factors involved in the production of slowly sedimenting nucleocapsids in measles virus-infected cells. J. Virol.37, 103–108 (1981).
Coto, C. E., Leon, M. E., Help, G. I.: Rol del genoma del huésped en la multiplicación del virus Junín (FHA). Acta Bqca. Clínica Latinoamericana9, 246–257 (1975).
Coto, C. E., Leon, M. E., Peralta, L. M., Help, G. I., Laguens, R. P.: Induction of infectious virus and viral surface antigen in Vero cells persistently infected with Junin virus. In:Karcher, D., Lowenthal, A., Strosberg, A. D. (eds.), Humoral Immunity and Neurological Diseases, 405–415. New York: Plenum Press 1979.
Damonte, E. B., Coto, C. E.: Temperature sensitivity of the arenavirus Junin isolated from persistently infected Vero cells. Intervirology11, 282–287 (1979).
Damonte, E. B., D'Aiutolo, A. C., Coto, C. E.: Persistent infection of Vero cells with Tacaribe virus. J. gen. Virol.56, 41–48 (1981).
Damonte, E. B., Mersich, S. E., Coto, C. E.: Response of cells persistently infected with arenaviruses to superinfection with homotypic and heterotypic viruses. Virology129, 474–478 (1983).
Francoeur, M., Stanners, C. P.: Cellular quiescence modulates the replication of Lts mutants of vesicular stomatitis virus. J. gen. Virol.50, 337–344 (1980).
Jacobson, S., Dutko, F. J., Pfau, C. J.: Determinants of spontaneous recovery and persistence in MDCK cells infected with lymphocytic choriomeningitis virus. J. gen. Virol.44, 113–121 (1979).
Kang, C. Y., Allen, R.: Host function-dependent induction of defective interfering particles of vesicular stomatitis virus. J. Virol.25, 202–206 (1978).
Lee, G. T. Y., Engelhardt, D. L.: Protein metabolism during growth of Vero cells. J. Cell. Physiol.92, 293–302 (1977).
Lehmann-Grube, F., Martinez Peralta, L., Bruns, N., Lohler, J.: Persistent infection of mice with the lymphocytic choriomeningitis virus. Compr. Virol.18, 43–103 (1983).
Mersich, S. E., Damonte, E. B., Coto, C. E.: Induction of RNA polymerase II activity in Junin virus infected cells. Intervirology16, 123–127 (1981).
Ogura, H., Sato, H., Hatano, M.: Relation of HVJ (Sendai virus) production to cell growth phase in persistently infected mouse 3T3 cells. Arch. Virol.80, 47–57 (1984).
Rawls, W. E., Chan, M. A., Gee, S. R.: Mechanisms of persistence in arenavirus infections: a brief review. Canad. J. Microbiol.27, 568–574 (1981).
Welsh, R. M., Buchmeier, M. J.: Protein analysis of defective interfering lymphocytic choriomeningitis virus and persistently infected cells. Virology96, 503–515 (1979).
Author information
Authors and Affiliations
Additional information
With 2 Figures
Member of Research Career from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET).
Rights and permissions
About this article
Cite this article
Candurra, N.A., Damonte, E.B. Influence of cellular functions on the evolution of persistent infections with Junin virus. Archives of Virology 86, 275–282 (1985). https://doi.org/10.1007/BF01309831
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309831