Summary
Fibronection is lost from the surface of HSV infected cells during cell rounding. In order to investigate also the fate of fibronection during the process of HSV-induced cell-fusion, BHK, Vero as well as primary or secondary rabbit kidney cells were infected with HSV-1 strains producing cell-fusion. By immunofluorescence and immunoelectron microscopy a considerable loss of fibronectin after HSV infection could be demonstrated leaving only irregular clumps of fibronectin containing virus particles on the cell surface. Decrease and disarrangement of fibronectin was similar during cell rounding and cell fusion. Loss of Fibronectin was closely connected with the two types of the cytopathic effect (CPE) and could not be prevented by protease inhibitors. The immediate-early protein 175K is essential for induction of CPE and loss of fibronectin. The damage to the cell membrane during HSV infection shows certain analogous mechanisms with events induced by Cytochalasin B and might be explained by the loss of hypothetical fibronectin receptors.
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Mullins, D. E., Rohrlich, S. T.: The role of proteinases in cellular invasiveness. BBA695, 177–214 (1983).
Dienes, H. P., Müller, S., Falke, D.: The influence of replicating HSV on the microfilaments of cells. (In preparation.)
Dargan, D., Subak-Sharpe, J. H.: Ultrastructural Characterization of Herpes simplex Virus Type 1 (Strain 17) Temperature-sensitive mutants. J. gen. Virol.64, 1311–1326 (1983).
Dienes, H. P., Ramadori, G., Falke, D., Thoenes, W.: Electron microscopic observations on primary hepatocyte cultures infected with Herpes simplex virus types 1 und 2. Virchows Arch. (Cell Pathol.)46, 321–332 (1984).
Dierich, M. P., Landen, B., Schulz, Th., Falke, D.: Protease activity on the surface of HSV-infected cells. J. gen. Virol.45, 241–244 (1979).
Geiger, B.: Membrane-Cytoskeleton Interaction. BBA737, 305–341 (1983).
Goertz, J., Brake, B., Härle-Grupp, V., Falke, D.: Replication of HSV-1 in murine peritoneal macrophages: Comparison of various virus strains with different properties. Arch. Virol.79, 173–187 (1984).
Heeg, U., Haase, W., Brauer, D., Falke, D.: Microtubules and microfilaments in HSV-infected rabbit kidney cells. Arch. Virol.70, 233–246 (1981).
Heeg, U., Hiller, G., Härle-Grupp, V., Falke, D.: The cytoskeleton of HSV-infected cells. Herpes viruses, Cold Spring Harbor 31. 8.–5. 9. 1982, p. 100 (1982).
Heeg, U., Hiller, G., Härle-Grupp, V., Falke, D.: Alterations of the cycloskeleton after infection with HSV. Workshop of Section Virology of Deutsche Ges. f. Hyg. and Microbiol., Würzburg 13.–17. März, Zbl. Bakt. I Orig.A 255, 171 (1983).
Howett, M. K., High, C. S., Rapp, F.: Production of plasminogen activator by cells transformed by herpes viruses. Cancer Res.38, 1075–1078 (1978).
Hynes, R. O.: Cell surface proteins and malignant transformation. BBA458, 73–107 (1976).
Julkuinen, J., Hautanen, A., Keski-Oja, J.: Interaction of viral envelope glycoproteins with Fibronectin. Infect. Immun.40, 876–881 (1982).
Kleinman, H. K., Martin, G. R., Fishman, P. H.: Ganglioside inhibition of fibronectin-mediated cell-adhesion to collagen. Proc. Natl. Acad. Sci. U.S.A.76, 3367–3371 (1979).
Kurkinen, M., Wartiovaara, J., Vaheri, A.: Cytochalasin B releases a major surface-associated glycoprotein, Fibronection, from cultured fibroblasts. Exp. Cell Res.111, 127–137 (1978).
Norrild, B., Virtanen, I., Lehto, V.-P., Pedersen, B.: Accumulation of Herpes simplex type 1 glycoprotein D in adhesion areas of infected cells. J. gen. Virol.64, 2499–2503 (1983).
Podleski, T. R., Greenberg, J., Schlessinger, J., Yamada, K. M.: Fibronectin delays the fusion of L6 myoblasts. Exp. Cell Res.122, 317–326 (1979).
Richter, I. E., Falke, D.: Scanning Electron Microscope Observations on the Inhibition of Herpes-induced giant cell formation by Cpd 48/80 and Cytochalasin B. Eur. Journ. Path.9, 59–73 (1974).
Stanislawski, L.: Attachment of Rous Sarcoma Virus to the Fibroncetin matrix of infected chick embryo fibroblasts. J. Ultrastruc. Res.82, 134–142 (1983).
Vaheri, A., Kurkinen, M., Lehto, V.-P., Linder, E., Timpl, R.: Codistribution of pericellular matrix proteins in cultured fibroblasts and loss in transformation: fibronectin and Procollagen. Proc. Natl. Acad. Sci. U.S.A.75, 4944–4948 (1978).
Vaheri, A., Mosher, D. F.: High Molecular weight, cell surface-associated glycoprotein (Fibronectin) lost in malignant transformation. BBA516, 1–25 (1978).
Vartio, T., Vaheri, A.: Fibronectin: Chains of domains with diversified functions. TIBS8, 442–444 (1983).
Weber, K., Rathke, P. C., Osborn, M., Franke, W. W.: Distribution of actin and tubulin in cells and in glycerinated cell models after treatment with cytochalasin B (CB). Exp. Cell Res.101, 285–297 (1976).
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Dienes, H.P., Knoblich, A. & Falke, D. Loss of surface fibronectin after infection of cultured cells by HSV-1 and 2. Archives of Virology 86, 223–237 (1985). https://doi.org/10.1007/BF01309827
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DOI: https://doi.org/10.1007/BF01309827