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Construction of an infectious DNA clone of the Y 1 strain of canine parvovirus and characterization of the virus derived from the clone

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Summary

We have cloned genome fragments of canine parvovirus strain Y 1 from replicative-form DNA and double-stranded DNA synthesized from virion DNA in vitro, and constructed a recombinant plasmid containing a full-length Y 1 genome (pCPVY 1). When this recombinant plasmid was transfected into cell cultures, an infectious virus could be recovered. To characterize this pCPVY 1-derived virus, its biological properties were compared with those of the parental strain. No difference was observed between them in antigen expression, viral DNA replication, hemagglutination ability, and virus multiplication, indicating that the virus derived from the infectious plasmid inherited the biological properties of the authentic Y 1 strain. Therefore, this recombinant plasmid appears to be useful for reverse genetics of canine parvovirus.

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References

  1. Antonietti J-P, Sahli R, Beard P, Hirt B (1988) Characterization of the cell type-specific determinant in the genome of minute virus of mice. J Virol 62: 552–557

    Google Scholar 

  2. Bloom ME, Alexandersen S, Garon CF, Mori S, Wei W, Perryman S, Wolfinbarger JB (1990) Nucleotide sequence of the 5′-terminal palindrome of aleutian mink disease parvovirus and construction of an infectious molecular clone. J Virol 64: 3551–3556

    Google Scholar 

  3. Carlson J, Rushlow K, Maxwell I, Maxwell F, Winston S, Hahn W (1985) Cloning and sequence of DNA encoding structural proteins of the autonomous parvovirus feline panleukopenia virus. J Virol 55: 574–582

    Google Scholar 

  4. Carmichael LE, Joubert JC, Pollock RVH (1980) Hemagglutination by canine parvovirus: serologic studies and diagnostic applications. Am J Vet Res 41: 784–791

    Google Scholar 

  5. Chejanovsky N, Carter BJ (1990) Mutation of a consensus purine nucleotide binding site in the adeno-associated virusrep gene generates a dominant negative phenotype for DNA replication. J Virol 64: 1764–1770

    Google Scholar 

  6. Crandell RA, Fabricant CG, Nelson-Rees WA (1973) Development, characterization, and viral susceptibility of a feline (felis catus) renal cell line (CRFK). In Vitro Cell Dev Biol 9: 176–185

    Google Scholar 

  7. Gardiner EM, Tattersall P (1988) Mapping of the fibrotropic and lymphotropic host range determinants of the parvovirus minute virus of mice. J Virol 62: 2605–2613

    Google Scholar 

  8. Goto H, Hirano T, Uchida E, Watanabe K, Shinagawa M, Ichijo S, Shimizu K (1984) Comparative studies of physicochemical and biological properties between canine parvovirus and feline panleukopenia virus. Jpn J Vet Sci 46: 519–526

    Google Scholar 

  9. Higashihara T, Izawa H, Onuma M, Kodama H, Mikami T, Noda H (1981) Mink enteritis in Japan. I. Isolation and characterization of the causative virus and its pathogenicity in cat. Jpn J Vet Sci 43: 841–851

    Google Scholar 

  10. Horiuchi M, Ishiguro N, Goto H, Shinagawa M (1992) Characterization of the stage(s) in the virus replication cycle at which the host-cell specificity of the feline parvovirus subgroup is regulated in canine cells. Virology 189: 600–608

    Google Scholar 

  11. Kariatsumari T, Horiuchi M, Hama E, Yaguchi K, Ishiguro N, Goto H, Shinagawa M (1991) Construction and nucleotide sequence analysis of an infectious DNA clone of the autonomous parvovirus, mink enteritis virus. J Gen Virol 72: 867–875

    Google Scholar 

  12. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685

    Google Scholar 

  13. Li X, Rhode SL III (1990) Mutation of lysine 405 to serine in the parvovirus H-1 NS 1 abolishes its functions for viral DNA replication, late promotertrans-activation, and cytotoxicity. J Virol 64: 4654–4660

    Google Scholar 

  14. Martyn JC, Davidson BE, Studdert MJ (1990) Nucleotide sequence of feline panleukopenia virus: comparison with canine parvovirus identifies host-specific differences. J Gen Virol 71: 2747–2753

    Google Scholar 

  15. Merchlinsky MJ, Tattersall PJ, Leary JJ, Cotmore SF, Gardiner EM, Ward DC (1983) Construction of an infectious molecular clone of the autonomous parvovirus minute virus of mice. J Virol 47: 227–232

    Google Scholar 

  16. Mochizuki M, Hashimoto T (1986) Growth of feline panleukopenia virus and canine parvovirus in vitro. Jpn J Vet Sci 48: 841–844

    Google Scholar 

  17. Mochizuki M, Konishi S, Ajiki M, Akaboshi T (1989) Comparison of feline parvovirus subspecific strains using monoclonal antibodies against a feline panleukopenia virus. Jpn J Vet Sci 51: 264–272

    Google Scholar 

  18. Parrish CR, Aquadro CF, Carmichael LE (1988) Canine host range and a specific epitope map along with variant sequences in the capsid protein gene of canine parvovirus and related feline, mink, raccoon parvoviruses. Virology 166: 293–307

    Google Scholar 

  19. Parrish CR (1990) Emergence, natural history, and variation of canine, mink, and feline parvoviruses. Adv Virus Res 38: 403–450

    Google Scholar 

  20. Parrish CR (1991) Mapping specific functions in the capsid structure of canine parvovirus and feline panleukopenia virus using infectious plasmid clones. Virology 183: 195–205

    Google Scholar 

  21. Reed AP, Jones EV, Miller TJ (1988) Nucleotide sequence and genome organization of canine parvovirus. J Virol 62: 266–276

    Google Scholar 

  22. Rhode SL III (1989) Both excision and replication of cloned autonomous parvovirus DNA require the NS 1 (rep) protein. J Virol 63: 4249–4256

    Google Scholar 

  23. Samulski RJ, Srivastava A, Berns KI, Muzyczka N (1983) Rescue of adeno-associated virus from recombinant plasmids: gene correction within the terminal repeats of AAV. Cell 33: 135–143

    Google Scholar 

  24. Senda M, Hirayama N, Yamamoto H, Kurata K (1986) An improved hemagglutination test for study of canine parvovirus. Vet Microbiol 12: 1–6

    Google Scholar 

  25. Senda M (1989) Studies on canine parvovirus hemagglutination (in Japanese). Ph.D. thesis. Azabu University, Sagamihara-shi, Kanagawa, Japan

    Google Scholar 

  26. Shinagawa M, Nomura Y, Kariatumari T, Ishiguro N, Horiuchi M, Goto H (1989) Characterization of replicative form DNA of the autonomous parvovirus mink enteritis virus. Microbiol Immunol 33: 721–732

    Google Scholar 

  27. Shull BC, Chen KC, Lederman M, Stout ER, Bates RC (1988) Genomic clones of bovine parvovirus: construction and effect of deletions and terminal sequence inversions on infectivity. J Virol 62: 417–426

    Google Scholar 

  28. Siegl G, Bate RC, Berns KI, Carter BJ, Kelly DC, Kurstak E, Tattersall P (1985) Characteristics and taxonomy ofParvoviridae. Intervirology 23: 61–73

    Google Scholar 

  29. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517

    Google Scholar 

  30. Tratschin J-D, McMaster GK, Kronauer G, Siegl G (1982) Canine parvovirus: relationship to wild-type and vaccine strains of feline panleukopenia virus and mink enteritis virus. J Gen Virol 61: 33–41

    Google Scholar 

  31. Tratschin J-D, Miller IL, Carter BJ (1984) Genetic analysis of adeno-associated virus: properties of deletion mutants constructed in vitro and evidence for an adeno-associated virus replication function. J Virol 51: 611–619

    Google Scholar 

  32. Truyen U, Parrish CR (1992) Canine and feline host ranges of canine parvovirus and feline panleukopenia virus: distinct host cell tropisms of each virus in vitro and in vivo. J Virol 66: 5399–5408

    Google Scholar 

  33. Vasudevacharya J, Basak S, Srinivas RV, Compans RW (1990) The complete nucleotide sequence of an infectious clone of porcine parvovirus, strain NADL-2. Virology 178: 611–616

    Google Scholar 

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Authors and Affiliations

  1. Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan

    M. Horiuchi & M. Shinagawa

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  1. M. Horiuchi
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  2. M. Shinagawa
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Horiuchi, M., Shinagawa, M. Construction of an infectious DNA clone of the Y 1 strain of canine parvovirus and characterization of the virus derived from the clone. Archives of Virology 130, 227–236 (1993). https://doi.org/10.1007/BF01309656

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  • DOI: https://doi.org/10.1007/BF01309656

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