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A rapid method for establishment of a reverse genetics system for canine parvovirus

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Abstract

Canine parvovirus (CPV) is an important and highly prevalent pathogen of dogs that causes acute hemorrhagic enteritis disease. Here, we describe a rapid method for the construction and characterization of a full-length infectious clone (rCPV) of CPV. Feline kidney (F81) cells were transfected with rCPV incorporating an engineered EcoR I site that served as a genetic marker. The rescued virus was indistinguishable from that of wild-type virus in its biological properties.

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Acknowledgements

This work was supported by 863 Project (2011AA10A213) and National Key Research and Development Program of China (2016YFD0501001).

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Authors and Affiliations

  1. State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China

    Yongle Yu, Jun Su, Jigui Wang, Ji Xi, Yaping Mao, Qiang Hou, Xiaomei Zhang & Weiquan Liu

Authors
  1. Yongle Yu
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  2. Jun Su
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  3. Jigui Wang
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  4. Ji Xi
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  5. Yaping Mao
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  6. Qiang Hou
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  7. Xiaomei Zhang
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  8. Weiquan Liu
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Contributions

Conceived and designed the experiments: Yongle Yu and Weiquan Liu. Performed the experiments: Yongle Yu. Analyzed the data: Yongle Yu. Contributed reagents/materials/analysis tools: Yongle Yu, Jun Su, Jigui Wang, Ji Xi, Yaping Mao, Qiang Hou, Xiaomei Zhang, and Weiquan Liu. Wrote the paper: Yongle Yu and Weiquan Liu.

Corresponding author

Correspondence to Weiquan Liu.

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Conflict of interest

No conflict of interest exists in the submission of this manuscript, and manuscript is approved by all authors for publication.

Ethical approval

All animal experiments were performed following protocols approved by the State Key Laboratory of Agrobiotechnology Animal Study Proposal of China Agricultural University.

Additional information

Edited by Zhen F. Fu.

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Yu, Y., Su, J., Wang, J. et al. A rapid method for establishment of a reverse genetics system for canine parvovirus. Virus Genes 53, 876–882 (2017). https://doi.org/10.1007/s11262-017-1497-0

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  • DOI: https://doi.org/10.1007/s11262-017-1497-0

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