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Genetic characterization of simian immunodeficiency virus isolated from an African mandrill

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Summary

We constructed an infectious molecular clone of simian immunodeficiency virus from an African mandrill (SIVMND). Upon transfection, this clone directed the production of progeny virus particles infectious to and cytopathic for CD4+ human leukemia cells. Thirteen frameshift proviral mutants with an alteration in the eight open reading frames of SIVMND were generated by recombinant DNA techniques, and were analyzed biologically and biochemically. While mutations in the structural genesgag, pol, andenv abolished viral growth and induction of cytopathology, mutants of thevif, vpr, andnef genes were fully biologically active. Of thetat andrev mutants, only onerev mutant grew in CD4+ cells with delayed kinetics. In reporter-based transient expression systems, transactivation potentials of thetat andrev mutants were evaluated. A mutant lacking 2nd coding exon oftat gene exhibitedtat activity similar to that of the wild type clone. The infectiousrev mutant was partially defective forrev gene activity.

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Authors and Affiliations

  1. Institute of Virus Research, Kyoto University, Kyoto, Japan

    H. Sakai, J. -I. Sakuragi, S. Sakuragi, R. Shibata, M. Hayami, A. Ishimoto & A. Adachi

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  1. H. Sakai
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  2. J. -I. Sakuragi
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  4. R. Shibata
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Sakai, H., Sakuragi, J.I., Sakuragi, S. et al. Genetic characterization of simian immunodeficiency virus isolated from an African mandrill. Archives of Virology 125, 1–14 (1992). https://doi.org/10.1007/BF01309624

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