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Molecular cloning and sequencing of the coat protein gene of a Nebraskan isolate of tobacco necrosis virus: the deduced coat protein sequence has only moderate homology with those of strain A and strain D

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Summary

A partial nucleotide sequence spanning the coat protein (CP) gene of a Nebraskan isolate of tobacco necrosis virus (TNV-NE) has been determined. The sequence contains at least four open reading frames (ORFs). The 5′-terminal ORF encodes a protein that has 86% and 38% homology with the polymerases of strains A (TNV-A) and D (TNV-D), respectively. The second and third ORFs probably encode 10.7 kDa and 6.2 kDa proteins (p 10.7 and p 6.2). These are respectively 90% and 96% amino acid homologous encoded by similar ORFs in TNV-A but only 26% and 20% homologous with those in TNV-D. The fourth 3′-proximal ORF encodes the 30.3 kDa CP. The amino acid sequence of TNV-NE CP is only 51% and 44% homologous to those of TNV-A and TNV-D, respectively. Thus, the CP genes of TNV-NE, TNV-A, and TNV-D are quite different. Like the sequences to the 5′ side of the CP gene, that of TNV-NE is more closely related to TNV-A than to TNV-D.

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Authors and Affiliations

  1. Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, Nebraska, USA

    L. Zhang

  2. Agricultural Research Service, U.S. Department of Agriculture, Lincoln, Nebraska, USA

    R. French & W. G. Langenberg

Authors
  1. L. Zhang
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  2. R. French
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  3. W. G. Langenberg
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Additional information

This study was a cooperative investigation of the ARS, USDA, and the Nebraska Agricultural Experiment Station, under Project 21-12. It is published as paper no. 10147, Journal Series, Nebraska Agricultural Experiment Station. The nucleotide sequence data reported in this paper have been entered in the GenBank databases under the accession number L 04261.

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Zhang, L., French, R. & Langenberg, W.G. Molecular cloning and sequencing of the coat protein gene of a Nebraskan isolate of tobacco necrosis virus: the deduced coat protein sequence has only moderate homology with those of strain A and strain D. Archives of Virology 132, 291–305 (1993). https://doi.org/10.1007/BF01309540

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  • DOI: https://doi.org/10.1007/BF01309540

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