Skip to main content
SpringerLink
Log in

Effect of vagal blockade on food- and hormone-stimulated release of pancreatic polypeptide and motilin in dogs

  • Original Articles
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

Vagal control of food- and hormone-stimulated release of pancreatic polypeptide (PP) and motilin was investigated in four conscious dogs by examining the effect of cryogenic vagosympathetic blockade. The postprandial PP response of 189±7 pM was totally, although reversibly, inhibited to 58±11 pM with the vagi blocked. Similarly, bombesin-, CCK-OP-, or neurotensin-stimulated PP release was abolished. Although the PP response to intraduodenal perfusion of an elemental diet was also reduced by blockade, the 52±15% inhibition was less than observed with the meal. In contrast to PP, plasma motilin fell after the meal from a fasting level of 128±16 pM to a nadir of 52±7 pM. Vagal blockade reversed this decline as plasma motilin rose to a peak of 121±18 pM with a pattern resembling the motilin response in the interdigestive state. This motilin increment during blockade was inhibited by atropine and by infusion of porcine PP. Plasma motilin also fell with the elemental diet, but this response was not affected by blockade. During infusion of bombesin, plasma motilin rose by 60±9 pM; vagal blockade augmented this increment twofold. Thus, the PP response to a meal and to hormonal stimulation is controlled by a vagal cholinergic excitatory pathway. However, intestinal release of PP is mediated in part by the vagus and in part by a vagally independent mechanism which may be neural or hormonal. Alternatively, vagal noncholinergic inhibition is a major mechanism modulating the motilin response after oral food but motilin release exclusively from intestinal nutriments is mediated by nonvagal, noncholinergic mechanisms. That vagal blockade enhances bombesin-stimulated motilin release further supports control by a vagal inhibitory pathway. Since atropine inhibited the blockade-mediated rise of motilin after a meal, nonvagal cholinergic pathways also play a role in the regulation of motilin but may predominate only in the interdigestive state.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Schwartz TW, Rehfeld JF, Stadil F, Larson LI, Chance RE, Moon N: Pancreatic polypeptide response to food in duodenal ulcer patients before and after vagotomy. Lancet 1:1102–1105, 1976

    Google Scholar 

  2. Taylor IL, Feldman M, Richardson CT, Walsh JH: Gastric and cephalic stimulation of human pancreatic polypeptide release. Gastroenterology 75:432–437, 1978

    Google Scholar 

  3. Schwartz TW, Girotzinger H, Schoon IM, Stenguist B, Olbe L: Cephalic-vagal and vago-vagal stimulation of pancreatic polypetide (PP) secretion in man. Scand J Gastroenterol 14:313–320, 1979

    Google Scholar 

  4. Adrian TE, Bloom SR, Besterman HS, Barnes AJ, Cooke TJC, Russel RCG, Faber RG: Mechanisms of pancreatic polypeptide release. Lancet 1:161–163, 1977

    Google Scholar 

  5. Taylor IL, Impicciatore M, Carter DC, Walsh JH: Effect of atropine and vagotomy on pancreatic polypeptide response to a meal in dogs. Am J Physiol 235:E443–447, 1978

    Google Scholar 

  6. Taylor IL, Singer M, Kauffman GL: Time-dependent effects of vagotomy on pancreatic polypeptide release. Dig Dis Sci 27:491–494, 1982

    Google Scholar 

  7. Meyer FD, Gyr K, Hacki WH, Beglinger C, Jeker L, Varga L, Kayasseh L, Gillessen D, Stalder GA: The release of pancreatic polypeptide by CCK-octapeptide and some analogues in the dog. Gastroenterology 80:742–747, 1981

    Google Scholar 

  8. Taylor IL, Walsh JH, Carter DC, Wood J, Grossman MI: Effect of atropine and bethanechol on the pancreatic polypeptide and gastrin responses to bombesin. Gastroenterology 77:714–718, 1979

    Google Scholar 

  9. Blackburn AM, Bloom SR, Edwards AV: Pancreatic endocrine responses to physiological changes in plasma neurotensin concentration in the calf. J Physiol 318:407–412, 1981

    Google Scholar 

  10. You CH, Chey WY, Lee KY: Studies on plasma motilin concentration and interdigestive motility of the duodenum in humans. Gastroenterology 79:62–66, 1980

    Google Scholar 

  11. Hall KE, Greenberg GR, El-Sharkawy TY, Diamant NE: Vagal control of migrating motor complex related peaks in canine plasma motilin, pancreatic polypeptide and gastrin. Can J Physiol Pharmacol 61:1289–1298, 1983

    Google Scholar 

  12. Hall KE, Diamant NE, El-Sharkawy TY, Greenberg GR: Effect of pancreatic polypeptide on canine migrating motor complex and plasma motilin. Am J Physiol 245:G178-G185, 1983

    Google Scholar 

  13. Lee KY, Kim MS, Chey WY: Effect of a meal and gut hormones on plasma motilin and duodenal motility in dog. Am J Physiol 238:G280-G283, 1980

    Google Scholar 

  14. Christofides ND, Bloom SR: Plasma motilin levels in duodenal ulcer and effect of truncal vagotomy and hypoglycemia. Experientia 34:809–810, 1970

    Google Scholar 

  15. Phillipson EA, Hickey RF, Bainton CR, Nadel JA: Effect of vagal blockade on regulation of breathing in conscious dogs. J Appl Physiol 29:475–479, 1970

    Google Scholar 

  16. Schwartz TW, Stenquist B, Olbe L, Stadil F: Synchronous oscillations in the basal secretion of pancreatic polypeptide and gastric acid: Depression by cholinergic blockade of pancreatic polypeptide concentrations in plasma. Gastroenterology 76:14–19, 1979

    Google Scholar 

  17. Taylor IL, Kaufman GL Jr, Walsh JH, Trout H, Chew P, Harmon JW: Role of the small intestine and gastric antrum in pancreatic polypeptide release. Am J Physiol 240:G387–391, 1981

    Google Scholar 

  18. Modlin IM, Jaffe BM, Albert D, Materia A, Crochelt R, Sank A: The role of the antrum in the modulation of plasma pancreatic polypeptide levels. J Surg Res 30:269–274, 1981

    Google Scholar 

  19. Modlin IM, Lane FC, Sank A, Jaffe BM: Failure of pancreatic polypeptide release by pancreatic transplants. Surg Forum 30:209–210, 1980

    Google Scholar 

  20. Debas HT, Taylor IL, Seal AM, Passaro EP Jr: Evidence for vagus-dependent pancreatic polypeptide-releasing factor in the antrum: Studies with the autotransplanted dog pancreas. Surgery 92(2):309–314, 1982

    Google Scholar 

  21. Modlin IM, Albert D, Crochelt R, Sank A: Evidence for an intestinal mechanism of pancreatic polypeptide release. Dig Dis Sci 26:587–590, 1981

    Google Scholar 

  22. Funakoshi A, Glowniak J, Owyang C, Vinik AI: Evidence for cholinergic mechanisms modulating plasma motilin-like immunoreactivity. J Clin Endocrinol Metab 56(6):1129–1134, 1983

    Google Scholar 

  23. Diamant NE, Hall K, Mui H, El-Sharkawy TY: Vagal control of the feeding motor pattern in the lower esophageal sphincter, stomach, and small intestine of the dog.In Gastrointestinal Motility. J Christensen (ed). New York, Raven Press, 1980, pp 365–370

    Google Scholar 

  24. Christofides ND, Bloom SR, Besterman HS, Adrian TE, Ghatei MA: Release of motilin by oral and intravenous nutrients in man. Gut 20:102–106, 1979

    Google Scholar 

  25. Hall KE, Greenberg GR, El-Sharkawy TY, Diamant NE: The relationship between porcine motilin-induced MMC-like activity, vagal integrity and endogenous motilin release in dogs. Gastroenterology 87:76–85, 1984

    Google Scholar 

  26. Heppell J, Becker JM, Kelly KA, Zinsmeister AR: Postprandial inhibition of canine enteric interdigestive myoelectric complex. Am J Physiol 244:G160-G164, 1983

    Google Scholar 

  27. Poitras P, Tasse D, LaPrise P: Stimulation of motilin release by bombesin in dogs. Am J Physiol 245:G249-G256, 1983

    Google Scholar 

  28. Greenberg GR, McCloy RF, Adrian TE, Chadwick VS, Baron JH, Bloom SR: Inhibition of pancreas and gallbladder by pancreatic polypeptide. Lancet 2:1280–1282, 1978

    Google Scholar 

  29. Code CF, Marlett JA: The interdigestive myoelectric complex of the stomach and small bowel of dogs. J Physiol (London) 246:289–309, 1975

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicine, University of Toronto, Room 6356 Medical Sciences Bldg., M5S 1A8, Toronto, Ontario, Canada

    G. R. Greenberg MD, B. Chan MSc, S. R. Nordgren MD & J. Alleyne

Authors
  1. G. R. Greenberg MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Chan MSc
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. R. Nordgren MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Alleyne
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by a grant from the Medical Research Council of Canada (MA 6763). Dr. Greenberg is a recipient of a Medical Research Council of Canada Scholarship.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Greenberg, G.R., Chan, B., Nordgren, S.R. et al. Effect of vagal blockade on food- and hormone-stimulated release of pancreatic polypeptide and motilin in dogs. Digest Dis Sci 30, 946–953 (1985). https://doi.org/10.1007/BF01308294

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01308294

Keywords

Search

Navigation