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Role of prostaglandin E2 in cholinergic-mediated glycoprotein synthesis in canine antrum

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Abstract

We studied the mechanism of cholinergic stimulation of mucin synthesis in canine antral explants, including the role of PGE2 as an intermediate messenger. Isolated antral mucosa was incubated with 10−5 M carbachol (Cb), 10−5 M indomethacin (IND), 10−5 M pirenzepine (PZ), 10−5 M Cb+10−5 M PZ, 10−5 M Cb+10−5 M IND, and 10−5 M IND +PGE2 (10−8, 10−7 and 10−6 M) in the presence or absence of [3H]glucosamine. After 24 hr, total glycoprotein synthesis was quantitated by Sepharose-4B chromatography and by 10% TCA/1%PTA precipitation with lipid extraction. PGE2 released into the media was measured by radioimmunoassay (RIA). Cb significantly increased total glycoprotein synthesis and produced a significant increase in PGE2 release. The increase in glycoprotein synthesis and the release of PGE2 was blocked by the addition of muscarinic antagonist PZ. The addition of IND significantly inhibited glycoprotein synthesis and almost entirely suppressed PGE2 secretion. IND also inhibited the effect of Cb on glycoprotein synthesis and PGE2 release. Moreover, PGE2 (10−6 and 10−7 M) significantly increased the glycoprotein synthesis in the canine stomach. This suggests the coordinate participation of PGE2-releasing cell population in modulation of glycoprotein synthesis in gastric mucosa.

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Authors and Affiliations

  1. Department of Internal Medicine, VA Medical Center, 2215 Fuller Road, 48105, Ann Arbor, Michigan

    Kenji Yoshimura MD, Eugene R. Kraus MS, Shuya Shimakura MD, James M. Scheiman MD &  C. Richard Boland MD

  2. University of Michigan School of Medicine, Ann Arbor, Michigan

    Kenji Yoshimura MD, Eugene R. Kraus MS, Shuya Shimakura MD, James M. Scheiman MD &  C. Richard Boland MD

Authors
  1. Kenji Yoshimura MD
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  2. Eugene R. Kraus MS
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  3. Shuya Shimakura MD
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  4. James M. Scheiman MD
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  5. C. Richard Boland MD
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Additional information

Supported in part by the Research Service of the Veterans Administration and grant R01-DK37989 and Michigan Gastrointestinal Peptide Research Center, 5P30-DK34933 awarded by the PHS, DHHS.

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Yoshimura, K., Kraus, E.R., Shimakura, S. et al. Role of prostaglandin E2 in cholinergic-mediated glycoprotein synthesis in canine antrum. Digest Dis Sci 37, 1045–1050 (1992). https://doi.org/10.1007/BF01300285

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  • DOI: https://doi.org/10.1007/BF01300285

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