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Acid-gastric antisecretory effect of the ethanolic extract from Arctium lappa L. root: role of H+, K+-ATPase, Ca2+ influx and the cholinergic pathway

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Abstract

Background

Arctium lappa L., popularly known as burdock, is a medicinal plant used worldwide. The antiulcer and gastric-acid antisecretory effects of ethanolic extract from roots of Arctium lappa (EET) were already demonstrated. However, the mechanism by which the extract reduces the gastric acid secretion remains unclear. Therefore, this study was designed to evaluate the antisecretory mode of action of EET.

Materials and methods

The effects of EET on H+, K+-ATPase activity were verified in vitro, whereas the effects of the extract on cholinergic-, histaminergic- or gastrinergic-acid gastric stimulation were assessed in vivo on stimulated pylorus ligated rats. Moreover, ex vivo contractility studies on gastric muscle strips from rats were also employed.

Results

The incubation with EET (1000 µg/ml) partially inhibited H+, K+-ATPase activity, and the intraduodenal administration of EET (10 mg/kg) decreased the volume and acidity of gastric secretion stimulated by bethanechol, histamine, and pentagastrin. EET (100–1000 µg/ml) did not alter the gastric relaxation induced by histamine but decreased acetylcholine-induced contraction in gastric fundus strips. Interestingly, EET also reduced the increase in the gastric muscle tone induced by 40 mM KCl depolarizing solution, as well as the maximum contractile responses evoked by CaCl2 in Ca2+-free depolarizing solution, without impairing the effect of acetylcholine on fundus strips maintained in Ca2+ -free nutritive solution.

Conclusion

Our results reinforce the gastric antisecretory properties of preparations obtained from Arctium lappa, and indicate that the mechanisms involved in EET antisecretory effects include a moderate reduction of the H+, K+-ATPase activity associated with inhibitory effects on calcium influx and of cholinergic pathways in the stomach muscle.

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Acknowledgements

This work received grants from Fundação Araucária (Call 311/2014). Luisa Mota da Silva received a fellowship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil).

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Authors and Affiliations

  1. Departamento de Farmacologia, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, PR, Brazil

    Luisa Mota da Silva, Ligia de Moura Burci, Priscila de Souza, Rita de Cássia Melo Vilhena de Andrade Fonseca da Silva, Eunice André & Maria Fernanda de Paula Werner

  2. Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade do Vale do Itajaí, Itajaí, SC, Brazil

    Luisa Mota da Silva & Priscila de Souza

  3. Departamento de Bioquímica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, PR, Brazil

    Sandra Crestani, Nessana Dartora, Lauro Mera de Souza & Thales Ricardo Cipriani

  4. Laboratório de Biologia Cardiovascular, Departmento de Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil

    José Eduardo da Silva-Santos

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  1. Luisa Mota da Silva
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Correspondence to Luisa Mota da Silva or Maria Fernanda de Paula Werner.

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da Silva, L.M., Burci, L.d., Crestani, S. et al. Acid-gastric antisecretory effect of the ethanolic extract from Arctium lappa L. root: role of H+, K+-ATPase, Ca2+ influx and the cholinergic pathway. Inflammopharmacol 26, 521–530 (2018). https://doi.org/10.1007/s10787-017-0378-4

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