Abstract
The effects of neurotensin, a tridecapeptide localized mainly in the gut, on pancreatic secretion are controversial. It is not clear whether the effects are mediated by direct or indirect effects. The present study was done to determine the effect of threshold doses of neurotensinin vivo in the rat as well asin vitro to clarify whether the action is direct or indirect. Forin vivo studies the pancreatic duct of rats was cannulated and threshold doses of neurotensin and CCK-8 alone or in combination were infused as an intravenous bolus and pancreatic juice collected at 10-min intervals for 1 hr. Forin vitro studies dissociated lobules and acini were incubated for 1 hr with 10−11–10−6 M neurotensin with or without 10−9 M CCK-8 (maximally effective dose). Amylase secretion in the pancreatic juice or in the medium was determined. Neurotensin and CCK-8 at a dose of 50 pM/kg increased pancreatic protein secretionin vivo. Neurotensin in combination with CCK-8 did not potentiate protein secretion. In the pancreatic lobules and acini, neurotensin 10−11–10−6 M alone or in combination with CCK-8 (10−9 M) did not stimulate amylase secretion above the basal level or that stimulated with CCK-8. The combined data indicate that the effect of neurotensin is not mediated by a direct action on the pancreatic acinar cells.
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This work was supported by the Medical Research Service of the Veterans Administration.
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Singh, M., Bandisode, M.S. Effect of neurotensin on amylase secretion from rat pancreasin vivo andin vitro . Digest Dis Sci 32, 65–70 (1987). https://doi.org/10.1007/BF01296689
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DOI: https://doi.org/10.1007/BF01296689