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Hepatic bile formation in the rat

Addition of vasoactive intestinal peptide to the equation

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Abstract

While changes in gastric, pancreatic, and intestinal secretion in response to more recently identified gastrointestinal peptides have been characterized, there has been less investigation into effects of these hormones on hepatic bile production. The isolated perfused rat liver model has been used to examine effects of vasoactive intestinal peptide (VIP), somatostatin, bombesin, and thyrotropin-releasing hormone (TRH) on bile flow and bile acid transport. No changes were seen following bolus administration of bombesin (3×10−8–1.5×10−6 M) or TRH (3×10−7–3×10−6 M), while somatostatin (6×10−6 M) produced a small decrease in bile flow without any change in bile acid output. VIP (3×10−7 M) caused a highly significant increase in both volume of bile flow (0.85±0.8 to 1.11±0.09 μl/min/g liver,P<0.001) and bile acid output (31.6±1.5 to 43.2±1.7 nmol/min/g liver,P<0.001). Elimination of Ca2+ from liver perfusate did not prevent VIP-induced increases in bile flow and bile acid output, and no synergistic effect of concomitant theophylline administration was observed. While effects of VIP on bile flow appear to be due to alterations in hepatic transport of bile acids, the exact mechanism(s) producing these changes remains to be elucidated.

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Authors and Affiliations

  1. Gastroenterology Section, VA Medical Center, Minneapolis, Minnesota

    Robert G. Knodell MD, Nadine M. Steele BA & Laura N. Stanley BA

Authors
  1. Robert G. Knodell MD
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  2. Nadine M. Steele BA
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  3. Laura N. Stanley BA
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Additional information

This work was supported by National Institutes of Health grant AM-32208 and the Research Service of the Veterans Administration.

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Knodell, R.G., Steele, N.M. & Stanley, L.N. Hepatic bile formation in the rat. Digest Dis Sci 32, 1290–1296 (1987). https://doi.org/10.1007/BF01296380

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  • DOI: https://doi.org/10.1007/BF01296380

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