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Microinjection of profilins from different sources into the green algaMicrasterias causes transient inhibition of cell growth

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Summary

Recombinant profilins from different sources (Betula verrucosa, Schizosaccharomyces pombe, Acanthamoeba castellani, or man) cause marked effects on cell growth and morphogenesis when microinjected into growing cells of the green algaMicrasterias denticulata. Whereas control injections with β-lactoglobulin only result in a slight delay of cell growth, when profilin is injected cell differentiation ceases and only resumes about 1 to 2 h after the injection, depending on the dose. The resulting cell does not show any malformations, but is reduced in size and retarded in differentiation compared to controls. As a consequence of the profilin microinjection the pattern of cytoplasmic streaming and cytoplasmic structure are also altered. Gelsolin, injected for comparison, leads to minor retardation of cell development but produces less marked effects than profilin. Microinjection of fluorescently labeled profilin shows even distribution throughout the cytoplasm and more intense fluorescence in the nucleus. Electron microscopical investigations of cells fixed immediately after profilin injection show a normal distribution of dictyosomes, ER cisternae, microtubules, and secretory vesicles compared to noninjected controls at the same developmental stage. Our results indicate that disturbance of the natural actin turnover by the injection of actin-binding proteins strongly affects development ofMicrasterias, corroborating a key role of actin in the morphogenetic process.

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Authors and Affiliations

  1. Institut für Pflanzenphysiologie, Universität Salzburg, Hellbrannerstrasse 34, A-5020, Salzburg, Austria

    A. Holzinger & U. Meindl

  2. Institute of General and Experimental Pathology, General Hospital, University of Vienna, Vienna

    I. Mittermann, S. Laffer & R. Valenta

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Holzinger, A., Mittermann, I., Laffer, S. et al. Microinjection of profilins from different sources into the green algaMicrasterias causes transient inhibition of cell growth. Protoplasma 199, 124–134 (1997). https://doi.org/10.1007/BF01294501

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