Summary
The proportion of chromatin in the condensed state was determined in various tissues of five plant species. InVicia faba the influence of treatments with inhibitors of transcription and several growth temperatures was studied. No significant differences could be found except inPhaseolus vulgaris, where cell aging may contribute to some variation in chromatin organization, and in the highly specialized antipodal cells ofMelandrium dioicum, which exhibited an atypical nuclear ultrastructure. These quantitative data confirm earlier suggestions (Nagl 1979 a) that condensed chromatin in angiosperms is fairly species-specific, but not tissue-specific or function-related.
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References
Barlow, P., 1977: Determinants of nuclear chromatin structure in Angiosperms. Ann. Sci. Nat., Bot. Biol. Végét., Ser. 12,18, 193–206.
-Barlow, S., 1980: Nuclear structures and cell differentiation within a root apex. Ann. Rep. Austr. Natl. Univ. Sch. Biol. Sci., 42.
—,Hines, E. R., 1981: Chromatin organization and cell differentiation in the root apex of maize. Ann. Rep. ARC Letcombe Lab.1980, 70.
Brasch, K., Sinclair, G. D., 1978: The organization, composition and matrix of hepatocyte nuclei exposed to α-amanitin. Virchows Arch. B Cell Pathol.27, 193–204.
Derenzini, M., Pession-Brizzi, A., Betts-Eusebi, C., Novello, F., 1981: Relationship between the fine structural organization of chromatin and nucleic acid synthesis in regenerating rat hepatocytes. J. Ultrastruct. Res.75, 229–242.
Dhillon, S. S., Miksche, J. P., 1982: DNA content and heterochromatin variations in various tissues of peanut (Arachis hypogaea). Amer. J. Bot.69, 219–226.
Drumm, A., Nagl, W., 1982: Loss of DNA and euchromatin in senescing leaf cells ofAllium. Mech. Ageing Devel.18, 103–110.
Greimers, R., Deltour, R., 1981: Organization of transcribed and nontranscribed chromatin in isolated nuclei ofZea mays root cells. Eur. J. Biochem.23, 303–311.
Hirahara, S., 1980: Karyomorphological studies on somatic tissues inSpiranthes sinensis. J. Sci. Hiroshima Univ., Ser. B, Div. 2,17, 9–49.
Hsu, T. C., 1962: Differential rate in RNA synthesis between euchromatin and heterochromatin. Exp. Cell Res.27, 332–334.
Jeanny, J. C., Gontcharoff, M., 1978: Etude en microscopie électronique et par cytophotométrie à balayage de la structure et de la distribution de la chromatine dans les noyaux des cellules cartilagineuses deTriturus cristatus âgés au cours de la régénération. Wilhelm Roux's Arch.184, 195–211.
— —, 1981: Analysis of the changes in the morphology and staining density characteristics of cartilagenous cell nuclei fromTriturus cristatus during aging and regeneration. J. Histochem. Cytochem.29, 1281–1288.
Kiefer, G., Kiefer, R., Moore, G. W., Salm, R., Sandritter, W., 1974: Nuclear images of cells in different functional states. J. Histochem. Cytochem.22, 569–576.
Lafontaine, J. G., 1974: Ultrastructural organization of plant cell nuclei. In: The cell nucleus, vol. 1 (Busch, H., ed.), pp. 149–185. New York: Academic Press.
Leber, B., Hemleben, V., 1979: Nucleosomal organization in active and inactive plant chromatin. Plant Syst. Evol. Suppl.2, 187–199.
Nagl, W., 1976: Zellkern und Zellzyklen. Stuttgart: Ulmer.
—, 1979a: Nuclear ultrastructure: condensed chromatin in plants is species-specific (karyotypical), but not tissue-specific (functional). Protoplasma100, 53–71.
—, 1979b: Condensed interphase chromatin in plant and animal cell nuclei: fundamental differences. Plant Syst. Evol., Suppl.2, 247–260.
—, 1979c: Differential DNA replication in plants: a critical review. Z. Pflanzenphysiol.95, 283–314.
—, 1980: Chromosomen. Berlin: Parey.
—, 1982: Condensed chromatin: species-specificity, tissue-specificity, and cell cycle-specificity, as monitored by scanning cytometry. In: Cell growth (Nicolini, C., ed.), pp. 171–218. New York: Plenum.
Nagl, W., Peschke, W., 1982: Automatische Diagnose der artspezifischen Kern-Ultrastruktur sowie von Zellzyklusstadien bei Pflanzen mit Hilfe des Bildanalysesystems Leitz T.A.S. Leitz-Mitt. Wiss. Techn.8, 29–32.
Sachs, L., 1972: Statistische Auswertemethoden, 3. Aufl. Berlin: Springer.
Spurr, A. R., 1969: A low viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res.26, 31–43.
Sugita, M., Sasaki, K., 1982: Transcriptional activation and structural alteration of wheat chromatin during germination and seedling-growth. Physiol. Plant.54, 41–46.
Tanaka, A., 1982: Karyomorphological studies on somatic tissues inRaphanussativus. Mem. Fac. Educ. Ehime Univ., Ser. III,2, 11–77.
Tschermak-Woess, E., 1963: Strukturtypen der Ruhekerne von Pflanzen und Tieren. Wien: Springer.
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Nagl, W., Cabirol, H., Lahr, C. et al. Nuclear ultrastructure: Morphometry of nuclei from various tissues ofCucurbita, Melandrium, Phaseolus, Tradescantia, andVicia . Protoplasma 115, 59–64 (1983). https://doi.org/10.1007/BF01293581
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DOI: https://doi.org/10.1007/BF01293581