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Glutamate receptor activation induces carrier mediated release of endogenous GABA from rat striatal slices

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Summary

The regulation of striatonigral and striatopallidal GABAergic neurons by glutamatergic afferents is thought to play a critical role in normal basal ganglia function. Here we report that in striatal slices about 17% of K+-induced endogenous GABA release was Ca2+-independent and this could be blocked by a GABA transport inhibitor. Activation of N-methyl-D-aspartate (NMDA)- and quisqualate-sensitive receptors induced endogenous GABA efflux only in the presence of a GABA transaminase inhibitor; this efflux was inhibited by 60–80% with a GABA transport inhibiter. NMDA-induced GABA release was blocked by phencyclidine, Mg2+ and CGS 19755. Quisqualate-induced GABA release was blocked completely by a combination of the metabotropic antagonist, L-AP3 and CNQX, a non-NMDA receptor antagonist. These data indicate that excitatory amino acid agonists-induced GABA release is distinct from that induced by high K+ depolarization.

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  1. K. M. Johnson

    Present address: Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas, USA

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  1. Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas, USA

    J. Wang & G. Lonart

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  2. G. Lonart
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Wang, J., Lonart, G. & Johnson, K.M. Glutamate receptor activation induces carrier mediated release of endogenous GABA from rat striatal slices. J. Neural Transmission 103, 31–43 (1996). https://doi.org/10.1007/BF01292614

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  • DOI: https://doi.org/10.1007/BF01292614

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