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Three-dimensional study of cell organelles in the nutritive tissue of a gall (Liposthenes glechomae L. onGlechoma hederacea L.)

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Summary

Stereoscopic observation of thick sections makes possible the three-dimensional reconstruction of cell organelles. The material studied (nutritive tissue of the gall ofLiposthenes glechomae onGlechoma hederacea) was briefly fixed in glutaraldehyde and then impregnated with zinc iodide and osmium tetroxide. Thus it was possible to see the actual shape of the mitochondria. But above all, the selective impregnation of the material permitted the structural study of the cell's Golgi apparatus and lysosome system; in the nutritive tissue ofLiposthenes glechomae, participation in autophagy and cellular autolysis seems to be a main function of dictyosomes with a reticulotubular structure.

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References

  • Bronner, R., 1976: Contribution à l'étude histochimique des tissus nourriciers des zoocécidies. Thèse Doctorat d'Etat, Strasbourg.

  • Buvat, R., 1946: Action de l'eau sur le chondriome des cellules de la racine de chicorée à café. C. R. Acad. Sci. (Paris) (Ser. D)222, 970–971.

    Google Scholar 

  • —, 1961: La cellule végétale. L'univers des Connaissances, Paris: Hachette.

    Google Scholar 

  • —, 1977: Origine golgienne et lytique des vacuoles dans les cellules méristématiques des racines d'orge (Hordeum sativum). C. R. Acad. Sci. (Paris) (Ser. D)284, 167–170.

    Google Scholar 

  • Carasso, N., P.Favard, and R.Couteaux, 1975: Neurofibrils of the leech observed in HVM after silver impregnation. Proc. 4th Inter. Congr. High Voltage Electron Microscopy, Toulouse (Jouffrey, B., and P.Favard, eds.), pp. 381–384.

  • Coulomb, P., andC. Coulomb, 1972: Processus d'autophagie cellulaire dans les cellules de meristèmes radiculaires de la Courge (Cucurbita pepo L.) mis en état d'anoxie. C. R. Acad. Sci. (Paris) (Ser. D)274, 214–217.

    Google Scholar 

  • — —, 1973: Notion de GERL et d'appareil reticulaire interne de Golgi dans le méristème radiculaire de la Courge. C. R. Acad. Sci. (Paris) (Ser. D)277 1577–1580.

    Google Scholar 

  • — —, 1976: Appareil de Golgi et compartiment lysosomal. Ann. Sci. Nat. Bot. (Paris) 12ème Série,17, 309–320.

    Google Scholar 

  • Favard, P., L. Ovtracht, andN. Carasso, 1971: Observations de spécimens biologiques en microscope électronique à haute tension. I. Coupes épaisses. J. Microscopie,12, 301–316.

    Google Scholar 

  • Lechêne de la Porte, 1976: Différenciation des cellules de coiffe de pois et d'orge. Etude morphologique et cytochimique des modifications des dictyosomes. Ann. Sci. Nat. Bot. (Paris) 12ème Série,17, 345–350.

    Google Scholar 

  • Marty, F., 1973 a: Sites réactifs à l'iodure de zinc-tétroxyde d'osmium dans les cellules de la racine d'Euphorbia characias L. C. R. Acad. Sci. (Paris) (Ser. D)277, 1317–1320.

    Google Scholar 

  • —, 1973 b: Observation au microscope électronique à haute tension (3 MeV) de cellules végétales en coupes épaisses de 1 à 5μ. C. R. Acad. Sci. (Paris) (Ser. D)277, 2681–2684.

    Google Scholar 

  • Matile, P., 1975: Cell biology monographs. The lytic compartment of plant cells, pp. 183. Wien-New York: Springer.

    Google Scholar 

  • Novikoff, A. B., E. Essner, andN. Quintana, 1963: Relations of endoplasmic reticulum, Golgi apparatus and lysosomes. J. Microscop.2, 3.

    Google Scholar 

  • Novikoff, P. M., A. B. Novikoff, N. Quintana, andJ. J. Hauw, 1971: Golgi apparatus, GERL and lysosomes of neurones in rat dorsal root ganglio, studied by thick section and thin section cytochemistry. J. Cell. Biol.50, 859–880.

    Google Scholar 

  • Pellegrini, M., andL. Pellegrini, 1976: Continuité mitochondriale et discontinuité plastidale chezEuglena gracilis. C. R. Acad. Sci. (Paris) (Ser. D)282, 357–360.

    Google Scholar 

  • Poux, N., P. Favard, andN. Carasso, 1974: Etude en microscopie électronique haute tension de l'appareil vacuolaire dans les cellules méristématiques de racines de Concombre. J. Microscopie21, 173–180.

    Google Scholar 

  • Prat, R., B. Vian, D. Reis, andJ. C. Roland, 1977: Evolution of internal pressure, vacuolation and membrane flow, during cell growth in mung bean hypocotyl. Biol. Cellulaire28, 269–280.

    Google Scholar 

  • Rambourg, A., A. Marraud, andG. Thiery, 1974: Microscopie électronique à balayage par transmission: son intérêt pour l'étude tridimensionnelle des organites cellulaires. C. R. Acad. Sci. (Paris) (Ser. D)279, 283–284.

    Google Scholar 

  • Rohfritsch, O., 1974: Infrastructure du tissu nourricier de la galle deVAulax glechomae surGlechoma hederacea L. Protoplasma,81 205–230.

    Google Scholar 

  • Thiery, G., andA. Rambourg, 1976: New staining technique for studying thick sections in the electron microscope. J. Microscopie, Biol. Cell.26, 103–106.

    Google Scholar 

  • Vartapetian, B. B., I. N. Andreeva, G. I. Kozlova, andL. P. Agapova, 1977: Mitochondial ultrastructure in roots of mesophyte and hydrophyte at anoxia and after glucose feeding. Protoplasma,91, 243–256.

    Google Scholar 

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  1. Institut de Botanique, Equipe de Recherche du CNRS no 060050, associée à l'Université Louis Pasteur de Strasbourg, F-67083, Strasbourg Cedex, France

    Odette Rohfritsch

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  1. Odette Rohfritsch
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Rohfritsch, O. Three-dimensional study of cell organelles in the nutritive tissue of a gall (Liposthenes glechomae L. onGlechoma hederacea L.). Protoplasma 95, 297–307 (1978). https://doi.org/10.1007/BF01291406

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  • DOI: https://doi.org/10.1007/BF01291406

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