Summary
Mesophyll protoplasts ofNicotiana tabacum cv. Xanthi were subjected to centrifugation through a Percoll gradient. This resulted in the removal of the central vacuole from each protoplast, and improved mechanical and osmotic stability. Electron microscope studies showed that the remaining cell contents, including small vacuoles, were of normal morphology. Fixation of protoplasts at various times during subsequent culture showed that the central vacuole was restored after about 12 hours. Cell-wall formation was well advanced after 24 hours of culture. These results are discussed in terms of the potential use of evacuolate protoplasts and the mechanisms of vacuole formation.
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Abbreviations
- ER:
-
endoplasmic reticulum
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Burgess, J., Lawrence, W. Studies of the recovery of tobacco mesophyll protoplasts from an evacuolation treatment. Protoplasma 126, 140–146 (1985). https://doi.org/10.1007/BF01287680
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DOI: https://doi.org/10.1007/BF01287680