Summary
The cytoplasm ofAlsidium cells contains unusual structures showing various fibrillar arrangements, either stacked rows of arcs or parallel fibrils in longitudinal view alternating with fibrils in cross-section. These regions, located exclusively in the cytoplasmic matrix, are highly proteinaceous and do not seem to be complexed with polysaccharides. Tilting observations under the electron microscope show that the appearance of arcs is an illusion and that the pattern fits the explanation given byBouligand and collaborators for various twisted biological structures.
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Anderson, W. A., André, J., 1968: The extraction of some cell components with pronase and pepsin from thin sections of tissue embedded in an epon-araldite mixture. J. Microscopie7, 343–354.
Bouligand, Y., 1965: Sur une architecture torsadée répandue dans de nombreuses cuticules d'Arthropodes. C. R. Acad. Sci. Paris261, 3665–3668.
—, 1971: Les orientations fibrillaires dans le squelette des Arthropodes. I. L'exemple des crabes, l'arrangement torsadé des strates. J. Microscopie11, 441–472.
—, 1972: Twisted fibrous arrangements in biological materials and cholesteric mesophases. Tissue and Cell4, 189–217.
—,Soyer, M.-O., Puiseux-Dao, S., 1968: La structure fibrillaire et l'orientation des chromosomes chez les Dinoflagellés. Chromosoma24, 251–287.
Brown, R. M., 1972: Algal viruses. In: Advances in virus research, Vol. 17 (Smith, K. M., Lauffer, M. A., Bang, F. B., eds.), pp. 243–277. New York: Academic Press.
Catesson, A.-M., Czaninski, Y., 1980: Evolution structurale et cytochimique des parois lors de la différenciation du phloème secondaire. Biol. Cell.38, 27 a.
Cavalli, C., 1981: Contribution à l'étude de la Mousse de Corse,Alsidium helminthochorton Kützing. Th. Doct. Univ. Pharmacie, Aix-Marseille 2.
Gibbs, A., Skotnicki, A. H., Gardiner, J. E., Walker, E. S., Hollings, M., 1975: A Tobamovirus of a green alga. Virology64, 571–574.
Gourret, J. P., 1975: Une étude ultrastructurale comparative des mycoplasmes phytopathogènes, des bactéries des nodules racinaires et des plastes. Th. Doct. ès-Sciences, Rennes.
—, 1981: Intérêt de la platine goniométrique pour l'étude d'analogues biologiques de cristaux-liquides à structure cholestérique. Bull. Soc. bot. Fr.128, Actual. bot. (1), 55–59.
Gubb, D., 1975: A direct visualization of helicoidal architecture inCarcinus maenas andHalocynthia papillosa by scanning electron microscopy. Tissue and Cell7, 19–32.
Hoffman, L. R., Hoffman, C. S., 1975: Zoospore formation inCylindrocapsa. Can. J. Bot.53, 439–451.
Livolant, F., Giraud, M. M., Bouligand, Y., 1978: Agoniometric effect observed in sections of twisted fibrous materials. Biol. Cell.31, 159–168.
Meyer, J., Michler, P., 1976: Observations ultrastructurales sur la cellule nourricière duSynchytrium mercurialis Fuck., et sur le développement de la paroi du sporocyste. Marcellia39, 155–168.
Mollenhauer, H. H., Totten, C., 1971: Studies on seeds. I-Fixation of seeds. J. Cell Biol.48, 386–394.
Monneron, A., Bernhard, W., 1966: Action de certaines enzymes sur des tissus inclus en épon. J. Microscopie5, 697–714.
Neville, A. C., Gubb, D. G., Crawford, R. M., 1976: A new model for cellulose architecture in some plant cell walls. Protoplasma90, 307–317.
—,Luke, B. M., 1971: A biological system producing a selfassembling cholesteric protein liquid crystal. J. Cell Sci.8, 93–109.
Pearlmutter, N. L., Lembi, C. A., 1978: Localization of chitin in algal and fungal cell walls by light and electron microscopy. J. Histochem. Cytochem.26, 782–791.
Pellegrini, M., Pellegrini, L., 1983: Particularités ultrastructurales des cellules végétatives de l'Alsidium helminthochorton Kützing (Rhodophycées, Rhodomélacées). Ann. Sc. Nat., Botanique5, 211–227.
Reis, D., Vian, B., Roland, J. C., 1978:In vitro andin vivo polysaccharide assembly. Ultrastructural and cytochemical study of growing plant cell wall components. 9th Int. Cong. Electr. Microsc. Toronto11, 434–435.
Reynolds, E. S., 1963: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–212.
Robinson, D. G., Herzog, W., 1977: Structure, synthesis and orientation of microfibrils. III. A survey of the action of microtubule inhibitors on microtubules and microfibril orientation inOocystis solitaria. Cytobiologie15, 463–474.
Roland, J. C.,Vian, B., 1976: La sécrétion des parois: quelques étapes caractéristiques. Soc. bot. Fr., Coll. Sécrét. Végét. 21–32.
——, 1979: The wall of the growing plant cell: its three-dimensional organization. Int. Rev. Cytol.61, 129–166.
Sargent, C., 1978: Differentiation of the crossed fibrillar outer epidermal wall during extension growth inHordeum vulgare L. Protoplasma95, 309–320.
Shikata, E., Galvez-e, G. E., 1969: Fine flexuous threadlike particles in cells of plants and insect hosts infected with rice Hoja Bianca Virus. Virology39, 635–641.
Spurr, A. R., 1969: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res.26, 31–43.
Thiéry, J. P., 1967: Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J. Microscopie6, 987–1018.
Vian, B., 1978: On the interpretation of twisted patterns in elongating plant cell wall: informations obtained with ultracryotomy. Protoplasma97, 379–385.
Wilson, H. R., Tollin, P., 1970:Narcissus mosaic virus liquid crystals. J. Ultrastruct. Res.33, 550–553.
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Pellegrini, M., Pellegrini, L. On the occurrence of twisted fibrillar structures in the cytoplasm of the red algaAlsidium helminthochorton (La Tourette) Kütz., ultrastructural and cytochemical observations. Protoplasma 126, 54–61 (1985). https://doi.org/10.1007/BF01287673
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DOI: https://doi.org/10.1007/BF01287673