Summary
Glycolate oxidase activity is demonstrated cytochemically with the CeCl3 technique in leaf peroxisomes ofNicotiana tabacum, Glycine max, Psychotria punctata and in unspecialized peroxisomes ofYucca torreyi roots. Reaction product deposition occurs throughout peroxisomal matrices, whereas nucleoid inclusion bodies, occurring in all four species, are cytochemically unreactive. We observed reactive and nonreactive microbodies within individual cells in these four plants despite prolonged incubation times and increased CeCl3 concentration. These results may reflect differences in glycolate oxidase content or peroxisomal differentiation within individual cells of a given tissue. We demonstrate substrate-independent cerium deposits in cell walls, cytoplasmic hoop-shaped structures and chloroplast thylakoids at extended incubation times or increased CeCl3 concentration, perhaps indicating the presence of endogenous H2O2. Elemental spectral analysis of electron-dense deposits with energy dispersive X-ray microanalysis using a STEM detects peaks generated from the L-series of cerium. SEM X-ray mapping for the Lα1 peak of cerium verifies peroxisomal localization of reaction product in thin sections.
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Kausch, A.P., Wagner, B.L. & Horner, H.T. Use of the cerium chloride technique and energy dispersive X-ray microanalysis in plant peroxisome identification. Protoplasma 118, 1–9 (1983). https://doi.org/10.1007/BF01284741
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DOI: https://doi.org/10.1007/BF01284741