Skip to main content
SpringerLink
Log in

Reprogramming of cells following wounding in pea (Pisum sativum L.) roots. II. The effects of caffeine and colchicine on the development of new vascular elements

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

Interference with the normal progression of the cell cycle by the drugs caffeine and colchicine does not prevent parenchyma cells in the cortex of pea roots from being reprogrammed to become tracheary and sieve elements following severance of the vascular cylinder of the root. The pattern of secondary wall deposition of the newly differentiated tracheary elements is highly aberrant in the presence of colchicine but is of normal appearance in the caffeinetreated roots. In each case, the new sieve elements have sieve plates and lateral sieve areas with callose deposits. Induction and redifferentiation are achieved in the absence of cell division and microtubules in colchicine-treated roots.

Bi- and multi-nucleate cells are produced by both drugs. Microtubules are still present in the caffeine-treated roots but cell plate formation is inhibited. The partitioning of the multinucleate cortical cells by the interdigitation of “free-growing” walls between the nuclei occurs in the presence of caffeine but not colchicine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barlow, P. W., 1969: Differences in response to colchicine by differentiating xylem cells in roots ofPisum. Protoplasma68, 79–83.

    PubMed  Google Scholar 

  • Becerra, J., 1977: Calcium and magnesium in plant cytokinesis and their antagonism with caffeine. Experimentia33, 1318–1319.

    Google Scholar 

  • —,López-Sáez, J. F., 1978: Effects of caffeine, calcium and magnesium on plant cytokinesis. Exp. Cell Res.111, 301–308.

    PubMed  Google Scholar 

  • Behnke, H.-D., Schulz, A., 1980: Fine structure, pattern of division, and course of wound phloem inColeus blumei. Planta150, 357–365.

    Google Scholar 

  • Borisy, G. G., Taylor, E. W., 1967: The mechanism of action of colchicine. Binding of colchicine-3H to cellular protein. J. Cell Biol.34, 525–534.

    PubMed  Google Scholar 

  • Bowes, B. G., Torrey, J. G., 1976: Ultrastructural changes in cells of pea root cortical explants culturedin vitro. Protoplasma90, 99–118.

    Google Scholar 

  • Comer, A. E., 1978: Pattern of cell division and wound vessel member differentiation inColeus pith explants. Plant Physiol.62, 354–359.

    Google Scholar 

  • Dalessandro, G., Roberts, L. W., 1971: Induction of xylogenesis on pith parenchyma explants ofLactuca. Amer. J. Bot.58, 378–385.

    Google Scholar 

  • Davidson, D., Armstrong, S. W., 1980: Cell cycle duration and time of DNA synthesis in binucleate cells induced inV. faba meristems by caffeine or isobutyl-methylxanthine. Protoplasma102, 281–293.

    Google Scholar 

  • —,Golding, B. G., 1978: Differential behaviour of sister nuclei in methylxanthine-induced binucleate cells. Exp. Cell Res.116, 450–454.

    PubMed  Google Scholar 

  • —,Pertens, E., 1978: Differences in volumes of sister nuclei in binucleate cells: evidence for asymmetry of mitosis. Can. J. Bot.56, 2363–2369.

    Google Scholar 

  • Dodds, J. H., 1980: The effect of 5-fluorodeoxyuridine and colchicine on tracheary element differentiation in isolated mesophyll cells ofZinnia elegans L. Z. Pflanzenphysiol.99, 283–285.

    Google Scholar 

  • —, 1981: Relationship of the cell cycle to xylem cell differentiation: A new model. Plant, Cell and Environment4, 145–146.

    Google Scholar 

  • —,Phillips, R., 1977: DNA and histone content of immature tracheary elements from cultured artichoke explants. Planta135, 213–216.

    Google Scholar 

  • Evans, L. S., 1979: Developmental aspects of roots ofPisum sativum influenced by the G2 factor from cotyledons. Amer. J. Bot.66, 880–886.

    Google Scholar 

  • Feldman, L. J., Torrey, J. G., 1977: Nuclear changes associated with cellular dedifferentiation in pea root cortical cells culturedin vitro. J. Cell Sci.28, 87–105.

    PubMed  Google Scholar 

  • Fosket, D. E., 1968: Cell division and the differentiation of woundvessel members in cultured stem segments ofColeus. Proc. Nat. Acad. Sci.59, 1089–1096.

    Google Scholar 

  • —, 1970: The time course of xylem differentiation and its relation to deoxyribonucleic acid synthesis in culturedColeus stem segments. Plant Physiol.46, 64–68.

    Google Scholar 

  • —,Miksche, J. P., 1966: Protein synthesis as a requirement for wound xylem differentiation. Physiol. Plant.19, 982–991.

    Google Scholar 

  • Fukada, H., Komamine, A., 1980 a: Establishment of an experimental system for the study of tracheary element differentiation from single cells isolated from the mesophyll ofZinnia elegans. Plant Physiol.65, 57–60.

    Google Scholar 

  • — —, 1980 b: Direct evidence for cytodifferentiation to tracheary elements without intervening mitosis in a culture of single cells isolated from the mesophyll ofZinnia elegans. Plant Physiol.65, 61–64.

    Google Scholar 

  • — —, 1981: Relationship between tracheary element differentiation and the cell cycle in single cells isolated from the mesophyll ofZinnia elegans. Physiol. Plant.52, 423–430.

    Google Scholar 

  • Fulcher, R. G., McCully, M. E., Setterfield, G., Sutherland, J., 1976: β-1,3-glucans may be associated with cell plate formation during cytokinesis. Can. J. Bot.54, 539–542.

    Google Scholar 

  • Fulton, C., Walsh, C., 1980: Cell differentiation and flagellar elongation inNaegleria gruberi. J. Cell Biol.85, 346–360.

    PubMed  Google Scholar 

  • Giménez-Martín, G., González-Fernández, A., López-Sáez, J. F., 1965: A method of labelling cells. J. Cell Biol.26, 305–309.

    PubMed  Google Scholar 

  • González-Fernández, A., Giménez-Martín, G., López-Sáez, J. F., 1970: Cytokinesis at prophase in plant cells treated with ethidium bromide. Exp, Cell Res.62, 464–467.

    Google Scholar 

  • Green, P. B., 1969: Cell morphogenesis. Ann. Rev. Plant Physiol.20, 365–394.

    Google Scholar 

  • Hammersley, D. R. H., Mccully, M. E., 1980: Differentiation of wound xylem in pea roots in the presence of colchicine. Plant Sci Letters19, 151–156.

    Google Scholar 

  • Hardham, A. R., Gunning, B. E. S., 1980: Some effects of colchicine on microtubules and cell division in roots ofAzolla pinnata. Protoplasma102, 31–51.

    Google Scholar 

  • —,McCully, M. E., 1982: Reprogramming of cells following wounding in pea (Pisum sativum L.) roots. I. Cell division and differentiation of vascular tissue. Protoplasma112, 143–151.

    Google Scholar 

  • Hepler, P. K., Fosket, D. E., 1971: The role of microtubules in vessel member differentiation inColeus. Protoplasma72, 213–236.

    Google Scholar 

  • Jones, M. G. K., Payne, H. L., 1978 a: Cytokinesis inImpatiens balsamina and the effect of caffeine. Cytobios20, 79–91.

    PubMed  Google Scholar 

  • — —, 1978 b: Early stages of nematode-induced giant-cell formation in roots ofImpatiens balsamina. J. Nematology10, 70–84.

    Google Scholar 

  • Juniper, B. E., Lawton, J. R., 1979: The effect of caffeine, different fixation regimes and low temperature on microtubules in the cells of higher plants. Planta145, 411–416.

    Google Scholar 

  • Kastern, W. H., Krishnakumaran, A., 1975: Reprogramming in the absence of DNA synthesis inGalleria larval epidermis. Cell Differentiation4, 45–53.

    PubMed  Google Scholar 

  • Libbenga, K. R., Torrey, J. G., 1973: Hormone-induced endoreduplication prior to mitosis in cultured pea root cortex cells. Amer. J. Bot.60, 293–299.

    Google Scholar 

  • López-Sáez, J. F., Risueño, M. C., Giménez-Martiń, G., 1966: Inhibition of cytokinesis in plant cells. J. Ultrastruct. Res.14, 85–94.

    Google Scholar 

  • Malawer, C. L., Phillips, R., 1979: The cell cycle in relation to induced xylem differentiation: tritiated thymidine incorporation in cultured tuber explants ofHelianthus tuberosus L. Plant Sci. Letters15, 47–55.

    Google Scholar 

  • Morrison, I. N., O'brien, T. P., 1976: Cytokinesis in the developing wheat grain; division with and without a phragmoplast. Planta130, 57–67.

    Google Scholar 

  • Newcomb, W., 1973: The development of the embryo sac of sunflowerHelianthus annum after fertilization. Can. J. Bot.51, 879–890.

    Google Scholar 

  • —,Fowke, L. C., 1973: The fine structure of the change from freenuclear to cellular condition in the endosperm of chiceedStellaria media. Bot. Gaz.123, 236–241.

    Google Scholar 

  • Pareyre, C., Lasselain, M.-J., Deysson, G., 1979: The mechanism of cytokinesis in plant cells: action of caffeine on the kinetics of a root meristem cell population. Cytobios26, 153–166.

    PubMed  Google Scholar 

  • Paul, D. C., Goff, Ch. W., 1973: Comparative effects of caffeine, its analogues and calcium deficiency on cytokinesis. Exp. Cell Res.78, 399–413.

    Google Scholar 

  • Phillips, R., 1981: Characterization of mitotic cycles preceding xylogenesis in cultured explants ofHelianthus tuberosus L. Ann. Bot.47, 785–792.

    Google Scholar 

  • Pickett-Heaps, J. D., 1967: The effects of colchicine on the ultrastructure of dividing plant cells, xylem wall differentiation and distribution of cytoplasmic microtubules. Devl. Biol.15, 206–236.

    Google Scholar 

  • —, 1969: Preprophase microtubule bands in some abnormal mitotic cells of wheat. J. Cell Sci.4, 397–420.

    Google Scholar 

  • Poste, G., Allison, A. C., 1971: Membrane fusion reaction: a theory. J. theor. Biol.32, 165–184.

    PubMed  Google Scholar 

  • Rana, M. A., Gahan, P. B., 1981: Early cytochemical identification of cortical cells stimulated to form xylem elementsin vitro in roots ofPisum sativum. Proc. R. micros. Soc.16, 246.

    Google Scholar 

  • Robbertse, J. R., McCully, M. E., 1979: Regeneration of vascular tissue in wounded pea roots. Planta145, 167–173.

    Google Scholar 

  • Roberts, L. W., Baba, S., 1968: IAA-induced xylem differentiation in the presence of colchicine. Plant and Cell Physiol.9, 315–321.

    Google Scholar 

  • Roper, W., 1976: Nuclear fusion and irregular cytokinesis in binucleate and tetraploid cells ofVicia faba after caffeine treatment. Experimentia32, 1260–1261.

    Google Scholar 

  • —,Roper, S., 1977: Centripetal wall formation in roots ofVicia faba after caffeine treatment. Protoplasma93, 89–100.

    Google Scholar 

  • Sachs, T., 1978: Patterned differentiation in plants. Differentiation11, 65–73.

    Google Scholar 

  • Selman, K., Kafatos, F. C., 1974: Transdifferentiation in the labial gland of silk moths: is DNA required for cellular metamorphosis? Cell Differentiation,3, 81–94.

    PubMed  Google Scholar 

  • Shininger, T. L., 1975: Is DNA synthesis required for the induction of differentiation in quiescent root cortical parenchyma? Devl. Biol.45, 137–150.

    Google Scholar 

  • —, 1979: The control of vascular development. Ann. Rev. Plant Physiol.30, 313–337.

    Google Scholar 

  • Sinnott, E. W., Bloch, R., 1945: The cytoplasmic basis of intercellular patterns in vascular differentiation. Amer. J. Bot.32, 151–156.

    Google Scholar 

  • Torrey, J. G., 1975: Tracheary element formation from single isolated cells in culture. Physiol. Plant35, 158–165.

    Google Scholar 

  • Turgeon, R., 1975: Differentiation of wound vessel members without DNA synthesis; mitosis or cell division. Nature257, 806–808.

    PubMed  Google Scholar 

  • Volker, H., 1972: Licht- und elektronenmikroskopische Untersuchungen zur Cytokinese an jungen Brutkörpern vonMarchantia polymorpha L. Protoplasma75, 139–153.

    Google Scholar 

  • Vonderhaar, B. K., Topper, Y. J., 1974: A role of the cell cycle in hormone-dependent differentiation. J. Cell Biol.63, 707–712.

    PubMed  Google Scholar 

  • Zalin, R., 1979: The cell cycle, myoblast differentiation and prostaglandin as a developmental signal. Devl. Biol.71, 274–288.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Carleton University, KlS 5 B 6, Ottawa, Canada

    A. R. Hardham & M. E. McCully

  2. Department of Developmental Biology, R. S. B. S., Australian National University, P. O. Box 475, 2601, Canberra City, A. C. T., Australia

    A. R. Hardham

Authors
  1. A. R. Hardham
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. E. McCully
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hardham, A.R., McCully, M.E. Reprogramming of cells following wounding in pea (Pisum sativum L.) roots. II. The effects of caffeine and colchicine on the development of new vascular elements. Protoplasma 112, 152–166 (1982). https://doi.org/10.1007/BF01284089

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01284089

Keywords

Search

Navigation