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Investigations of the turnover of the putative Cellulose-synthesizing particle “rosettes” within the plasma membrane ofFunaria hygrometrica protonema cells

I. Effects of monensin and cytochalasin B

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Summary

YoungFunaria protonemata were treated with Monensin (10−6 M) and Cytochalasin (CB) (2×10−5 M). The influence of the inhibitors on a) elongation growth, b) cell fine structure and c) particle “rosettes” within the plasma membrane after freeze fracture was observed. Monensin stopped cell growth, caused swelling of the mitochondria and plastids and inhibited the secretory activity of the Golgi apparatus within about 15 minutes. The number of rosettes in the PF of the plasma membrane was distinctly reduced after 4–5 minutes and decreased further to only very few after 30 minutes. The tip to base gradient in distribution was maintained for a long time. The effects were reversible, regeneration occurred within 3 hours. CB treatment showed no effect on elongation growth and cell fine structure. The number of rosettes, however, was strongly reduced within 3 minutes exposure time and their distribution was nearly uniform then. Number and tip to base gradient increased again after 6 minutes intoxication. The results are discussed in regard to the turn over of the rosettes.

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Abbreviations

CB:

Cytochalasin B

PF:

protoplasmic fracture face

F-vesicle:

flat vesicle

F-Actin:

filamentous actin

G-Ac-tin:

globular actin

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  1. Institut für Zellenlehre, Universität Heidelberg, Im Neuenheimer Feld 230, D-6900, Heidelberg, Federal Republic of Germany

    U. Rudolph & E. Schnepf

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  1. U. Rudolph
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  2. E. Schnepf
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Rudolph, U., Schnepf, E. Investigations of the turnover of the putative Cellulose-synthesizing particle “rosettes” within the plasma membrane ofFunaria hygrometrica protonema cells. Protoplasma 143, 63–73 (1988). https://doi.org/10.1007/BF01282960

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