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Experimental and ultrastructural studies on cell shape formation in the defect mutant cellmicrasterias thomasiana f. uniradiata

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Summary

Cell development and ultrastructure are studied in the defect mutant cellMicrasterias thomasiana f. uniradiata which lacks cell pattern at one side of the cell.

The ultrastructural studies reveal an uneven distribution of vesicles, preponderating at the normally growing side of the cell, as well as the presence of a special kind of dark vesicles.

By means of turgor reduction and treatment with chlorotetracycline and cycloheximide some processes involved in cell shape formation are pointed out and are compared with those already described for biradiateMicrasterias cells.

It is demonstrated that the asymmetric cell shape of the mutant cell is already determined at the early stage of bulb formation and is due to a unilateral growth during the later stages of development. The asymmetric arrangement of the growth areas during cell development of the mutant is expressed by an asymmetric distribution of primary wall accumulations induced by turgor reduction as well as by the presence of fluorescence zones after treatment with the Ca2+ -chelate probe chlorotetracycline at only one side of the cell. Inhibition of protein synthesis by cycloheximide during cell growth of the mutant leads to the formation of a characteristically reduced cell pattern (“anuclear type of development”) similar to that ofMicrasterias denticulata andMicrasterias thomasiana under the same conditions. Nevertheless, this cell pattern develops at only one side of the cell, indicating that the mutant does not have any information for cell pattern formation at the defective side.

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  1. Institut für Botanik der Universität Salzburg, A-5020, Lasserstrasse 39, Salzburg, Austria

    Ursula Meindl

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Meindl, U. Experimental and ultrastructural studies on cell shape formation in the defect mutant cellmicrasterias thomasiana f. uniradiata . Protoplasma 129, 74–87 (1985). https://doi.org/10.1007/BF01282307

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