Skip to main content
SpringerLink
Log in

Plant regeneration from cotyledonary protoplasts of cauliflower (Brassica oleracea L. var.botrytis L.)

  • Brief Report
  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

Protoplasts isolated enzymatically from precultured cotyledonary leaves ofB. oleracea var.botrytis and cultured in KM8p medium (Kao andMichayluk 1975) underwent sustained divisions in about 0.1% population to eventually produce callus, whereas mesophyll protoplasts from either field grown orin vitro raised plants failed to divide. The callus readily differentiated on Murashige-Skoog medium as modified for shoot culture (Binding 1974) to give rise to shoot and roots.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Binding, H., 1974: Cell cluster formation by leaf protoplasts from axenic cultures of haploidPetunia hybrida L. Plant Sci. Lett.2, 185–188.

    Google Scholar 

  • Gatenby, A. A., Cocking, E. C., 1977: Callus formation from protoplasts of marrow stem kale. Plant Sci. Lett.8, 275–280.

    Google Scholar 

  • Kao, K. N., Michayluk, M. R., 1975: Nutritional requirements of growth ofVicia hajastana cells and protoplasts at very low density in liquid media. Planta (Berl.)126, 105–110.

    Google Scholar 

  • Kartha, K. K., Michayluk, M. R., Kao, K. N., Gamborg, O. L., Constabel, F., 1974: Callus formation and plant regeneration from mesophyll protoplasts of rape plants (Brassica napus L. cv. Zephyr). Plant Sci. Lett.3, 265–271.

    Google Scholar 

  • Murashige, T., Skoog, F., 1962: A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant.15, 473–497.

    Google Scholar 

  • Ohyama, K., Nitsch, J. P., 1972: Flowering haploid plants obtained from protoplasts of tobacco leaves. Plant Cell Physiol.13, 229–236.

    Google Scholar 

  • Sacristan, M. D., Melchers, G., 1969: The caryological analysis of plants regenerated from tumorous and other callus cultures of tobacco. Mol. gen. Genet.105, 317–333.

    Google Scholar 

  • Schenck, H. R., Hoffmann, F., 1979: Callus and root regeneration from mesophyll protoplasts of basicBrassica species:B. campestris, B. oleracea andB. nigra. Z. Pflanzenphysiol.82, 354–360.

    Google Scholar 

  • Shepard, J. F., 1981: Protoplasts as sources of disease resistance in plants. Ann. Rev. Phytopathol.19, 145–166.

    Google Scholar 

  • Ulrich, T. H., Chowdhury, J. B., Widholm, J. M., 1980: Callus and root formation from mesophyll protoplasts ofBrassica rapa. Plant Sci. Lett.19, 347–354.

    Google Scholar 

  • Vatsya, B., Bhaskaran, S., 1981: Production of sub-protoplasts inBrassica oleracea var.capitata: a function of osmolarity of the media. Plant Sci. Lett.23, 277–282.

    Google Scholar 

Download references

Author information

Author notes

  1. Bhartendu Vatsya

    Present address: Hindustan Lever Research Centre, 400 099, Chakala, Andheri East, Bombay, India

Authors and Affiliations

  1. Nuclear Research Laboratory, Indian Agricultural Research Institute, New Delhi

    Bhartendu Vatsya & S. Bhaskaran

Authors
  1. Bhartendu Vatsya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Bhaskaran
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vatsya, B., Bhaskaran, S. Plant regeneration from cotyledonary protoplasts of cauliflower (Brassica oleracea L. var.botrytis L.). Protoplasma 113, 161–163 (1982). https://doi.org/10.1007/BF01282006

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01282006

Keywords

Search

Navigation