Summary
The active sites of actin of oneCharaceae species were found to interact with the endoplasmic factor from a different species. Protoplasm was suqueezed out of cells ofChara australis with vacuoles that had been perfused beforehand with a medium containing EGTA and Mg · ATP. Centrifugation of this protoplasmic mixture divided it into the supernatant composed of endoplasmic granules and the precipitate composed of chloroplasts and nuclei. When the endoplasmic granular aggregates were introduced into a tonoplast-freeNitella axilliformis cell treated with NEM to inactivate the endoplasmic factor, they became attached to theNitella gel and streamed longitudinally with the polarity. Treatment of the endoplasmic granules with the strong Mg2+chelator CyDTA (1,2-cyclohexane diamineN, N′-tetraacetic acid) irreversibly inhibited reconstitution of the cytoplasmic streaming.
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Abbreviations
- APW:
-
artificial pond water
- ATP:
-
adenosine-5′-triphosphoric acid
- CyDTA:
-
cyclohexanediamine-N,N′-tetraacetic acid
- DTT:
-
dithiothreitol
- EGTA:
-
ethyleneglycol-bis-(β-aminoethylether)-N,N′-tetraacetic acid
- HMM:
-
heavy meromyosin
- NEM:
-
N-ethylmaleimide
- PEP:
-
phosphoenolypyruvate
- PIPES:
-
piperazine-N,N′-bis-(2-ethane-sulfonic acid)
- PK:
-
pyruvate kinase
- PMSF:
-
phenylmethylsulfonylfluoride
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Shimmen, T., Tazawa, M. Reconstitution of cytoplasmic streaming inCharaceae . Protoplasma 113, 127–131 (1982). https://doi.org/10.1007/BF01282001
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DOI: https://doi.org/10.1007/BF01282001