Summary
The influence of a morphactin, chlorflurenol-methylester (CFM), on the growth, the morphogenesis and the isoelectric peroxidase pattern was investigated in both callus cultures (two different tissue culture strains) and multiple bud cultures ofNicotiana tabacum var.Wisconsin. CFM (range of concentration between 10−6g/ml and 10−4g/ml) was applied singly, or in combination with a cytokinin, benzylaminopurine (BAP), or with an auxin, indoleacetic acid (IAA), or with IAA plus BAP.
In general, the callus growth was inhibited under the influence of CFM. In some of the experiments carried out in hormone-free media, growth stimulation was observed. Even minimal inhibition or stimulation of the callus growth was always accompanied by characteristic changes in the peroxidase patterns.
The following results show the influence of the morphactin CFM on cytokinin effects (endogenous cytokinin or equally the exogenously applied cytokinin, BAP). (1) In the multiple bud cultures, BAP and CFM (both substances combined with IAA) similarly caused inhibition of root formation and stimulation of bud formation. The bands in the peroxidase patterns, characteristic of cytokinin action, were accentuated also of those bud cultures which had been treated with BAP or with CFM. (2) In the callus cultures, the cytokinin characteristics appeared under CFM influence in the peroxidase patterns of one of the tissue culture strains only when CFM was applied in combination with BAP and not in combinations of CFM with IAA.
The observed morphactin-induced increase in the cytokinin effects could occur via changes in the hormone level of the tissue.
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References
Chawan, D. D., Sen, D. N., 1971: Antagonism between morphactin and cytokinins in the seedling growth ofLactuca sativa var.New York. Biochem. Physiol. Pflanzen162, 462–465.
Davidonis, G. H., Hamilton, R. H., Mumma, R. O., 1978: Metabolism of 2,4-Dichlorophenoxyacetic acid in soybean root callus and differentiated soybean root cultures as a function of concentration and tissues age. Plant Physiol.62, 80–83.
Delinceé, H., Radola, B. J., 1972: Detection of peroxidase by the print technique in thin-layer isoelectric focusing. Anal. Biochem.48, 536–545.
Feung, C. S., Hamilton, R. H., Mumma, R. O., 1977: Metabolism of indole-3-acetic acid. Plant Physiol.59, 91–93.
Harada, H., 1969: Some interactions between morphactin and growth substances. Ber. dtsch. bot. Ges. Neue Folge3, 77–88.
Koda, Y., Okazawa, Y., 1980: Cytokinin production byAsparagus shoot apex culturedin vitro. Physiol. Plant.49, 193–197.
Linsmaier, E. M., Skoog, F., 1965: Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant.18, 100–127.
Mäder, M., Bopp, M., 1976: Neue Vorstellungen zum Problem der Isoperoxidasen anhand der Trennung durch Disk-Elektro-phorese und isoelektrische Fokussierung. Planta (Berl.)128, 247–253.
Moore, F. H., 1979: A cytokinin-binding protein from wheat germ. Plant Physiol.64, 594–599.
Nessel, A., Mäder, M., 1977: Über die physiologische Bedeutung der Peroxidase-Isoenzymgruppen des Tabaks anhand einiger biochemischer Eigenschaften. Z. Pflanzenphysiol.82, 235–246.
Ogura, H., 1975: The role of morphactin in cultured tissues. Naturwissenschaften62, 393.
Parups, E. V., 1980: Effects of morphactin on certain plant growth substances in bean roots. Physiol. Plant.49, 281–285.
Radola, B. J., 1973: Isoelectric focusing in layers of granulated gels. Biochim. biophys. Acta295, 412–428.
Rücker, W., Markotai, J., 1977: Growth and isoelectric patterns of peroxidase in tobacco tissue cultures under the influence of growth regulator systems. Electrofocusing and Isotachophoresis, pp. 213–220. Berlin-New York: Walter de Gruyter & Co.
—, 1978: Gewebewachstum und isoelektrische Peroxidasemuster bei Tabakgewebekulturen unter dem Einfluß von Cytokinin, verschiedenen Phenylcarbonsäuren und aromatischen Aminsäuren. Phyton.19, Fase. 1–2, 1–11.
—, 1980: Isoelectric peroxidase patterns as indicators for growth and for the effect of growth regulators in tobacco tissue cultures. Electrophoresis '79, pp. 833–840. Berlin-New York: Walter de Gruyter & Co.
Schneider, G., 1969: Morphaktine, Wirkung auf Entwicklung und Wachstum von höheren Pflanzen. Ber. dtsch. bot. Ges. Neue Folge3, 19–41.
—, 1970: Morphactins: physiology and performance. Ann. Rev. Plant Physiol.21, 499–536.
Sembdner, G., Gross, D., Liebisch, H. W., Schneider, G., 1980: Hormonal regulation of development I. Biosynthesis and metabolism of plant hormones, pp. 360–371. Berlin-Heidelberg-New York: Springer.
Ziegler, H., Vogt, I., Treichel, S., Streitz, B., 1969: Zur physiologischen Wirkung der Morphaktine. Ber. dtsch. bot. Ges. Neue Folge3, 43–51.
Ziegler, H., 1970: Morphaktine. Endeavour29, 112–116.
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Rücker, W. Morphactin-induced changes in the cytokinin effect on tissue and organ cultures ofNicotiana tabacum . Protoplasma 113, 103–109 (1982). https://doi.org/10.1007/BF01281998
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DOI: https://doi.org/10.1007/BF01281998