Summary
The new technique of fluorescent analog cytochemistry was used to investigate the cell surface morphology (RITC-WGA staining), the organization of the microfilament system (Rh-phalloidin staining) and the spatial distribution of mitochondria (Rh-123 staining) in the various growth stages of axenically cultured living and fixed microplasmodia ofPhysarum polycephalum. The differentiation degree of the cell surface is generally size- and age-dependent: the invagination system develops by degrees from small spherical stages (50–100 μm) without invaginations to large vein-like or dumbbell-shaped specimens (300–1,000 (μm long) with extensive invagination systems. The microfilaments are always organized in a cortical system along the entire cell surface and sometimes in a fibrillar system as well, extending throughout the cytoplasmic matrix. Results on living microplasmodia demonstrate that the cortical microfilament system is mainly involved in motive force generation for changes of cell surface morphology and protoplasmic streaming activity, whereas the fibrillar system rather serves a stabilizing and adhering function. Moreover, the functional differences of the two microfilament systems are indicated by the position of a large population of stationary mitochondria in close vicinity to the cell surface, thus pointing to a reasonable arrangement of the energy-supplying and energy-transforming system.
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References
Achenbach F, Achenbach U, Wohlfarth-Bottermann K-E (1979) Plasmalemma invaginations, contraction and locomotion in normal and caffeine-treated protoplasmic drops ofPhysarum. Eur J Cell Biol 20: 12–23
—,Wohlfarth-Bottermann K-E (1981) Morphogenesis and disassembly of the circular plasmalemma invagination system inPhysarum polycephalum. Differentiation 19: 179–188
Ambrose EJ (1972) Cell shapes and cell contacts. Acta Protozool 11: 9–21
Begg DA, Rodewald R, Rebhun LI (1978) The visualization of actin filament polarity in thin sections. Evidence for the uniform polarity of membrane-associated filaments. J Cell Biol 79: 846–852
Brix K,Stockem W (in preparation) Studies on microplasmodia ofPhysarum polycephalum. VII. Adhesion-dependent changes in the organization of the fibrillar actin system
Daniel JW, Rusch HP (1961) The pure culture ofPhysarum polycephalum on a partially defined soluble medium. J Gen Microbiol 25: 47–49
Faulstich H, Trischmann H, Mayer D (1983) Preparation of tetramefhylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis. Exp Cell Res 144: 73–82
Gawlitta W, Wolf KV, Hoffmann H-U, Stockem W (1980) Studies on microplasmodia ofPhysarum polycephalum. I. Classification and locomotion behavior. Cell Tissue Res 209: 71–86
Gray WD, Alexopoulus CJ (1968) Biology of the myxomycetes. Ronald Press, New York
Grebecka L, Hrebenda B (1979) Topography of cortical layer inAmoeba proleus as related to the dynamic morphology of moving cell. Acta Protozool 18: 493–502
Hoffmann H-U, Meyer R, Gawlitta W, Wolf KV (1981) Studies on microplasmodia ofPhysarum polycephalum. IV. Three-dimensional reconstruction of a dumbbell-shaped microplasmodium. Microsc Acta 85: 59–67
Ishikawa H, Bischoff R, Holtzer H (1969) Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J Cell Biol 43: 312–328
Jockusch BM (1983) Patterns of microfilament organization in animal cells. Mol Cell Endocrinol 29: 1–19
—,Füchtbauer A (1983) Organization and function of structural elements in focal contacts of tissue culture cells. Cell Motil 3: 391–397
Kessler D (1982) Plasmodial structure and motility. In:Aldrich HC, Daniel JW (eds) Cell biology ofPhysarum andDidymium. Academic Press, New York, pp 145–208
Korohoda W, Stockem W (1975) On the nature of hyaline zones in the cytoplasm ofAmoeba proleus. Microsc Acta 77: 129–141
Kukulies J, Stockem W (1985) Function of the microfilament system in living cell fragments ofPhysarum polycephalum as revealed by microinjection of fluorescent analogs. Cell Tissue Res 242: 323–332
— — (1986) Fluorescent analog cytochemistry of living cells. Zeiss Information 29: 13–21
—,Brix K, Stockem W (1985) Fluorescent analog cytochemistry of the actin system and cell surface morphology inPhysarum microplasmodia. Eur J Cell Biol 39: 62–69
-Brix K,Stockem W (in preparation) Studies on microplasmodia ofPhysarum polycephalum. VI. Functional analysis of the cortical and fibrillar actin system by fluorescent analog cytochemistry
Nachmias VT, Huxley HE, Kessler D (1970) Electron microscope observation on actomyosin and actin preparations fromPhysarum polycephalum, and on their interaction with heavy meromyosin subfragment I from muscle myosin. J Mol Biol 50: 83–90
Opas M, Kalnins J (1984) Microfilament distribution and adhesion patterns in cultured cells after glutaraldehyde-formaldehyde fixation. Eur J Cell Biol 53: 60–65
Perdue JF (1973) The distribution, ultrastructure, and chemistry of microfilaments in cultured chick embryo fibroblasts. J Cell Biol 58: 265–283
Rhea RP (1966 a) Electron microscopic observations on the slime moldPhysarum polycephalum with specific reference to fibrillar structures. J Ultrastruct Res 15: 349–379
— (1966 b) Microcinematographic, electron microscopic and electrophysiological studies on shuttle streaming in the slime moldPhysarum polycephalum. In:Pai Si et al (eds) Dynamics of fluids and plasmas. Academic Press, New York, pp 35–58
Spooner BS, Yamada KM, Wessels NK (1971) Microfilaments and cell locomotion. J Cell Biol 49: 595–613
Spurr AR (1969) A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26: 31–43
Stockem W (1979) Cell surface morphology and activity inAmoeba proteus andPhysarum polycephalum. Acta Protozool 18: 33–41
—,Hoffmann H-U, Gawlitta W (1982) Spatial organization and fine structure of the cortical filament layer in normal locomotingAmoeba proteus. Cell Tissue Res 221: 505–519
Usui N (1971) Fibrillar differentiation in a microplasmodium of the slime moldPhysarum polycephalum. Developm Growth Differ 13: 241–255
Wang YL, Heiple JM, Taylor DL (1982) Fluorescent analog cytochemistry of contractile proteins. In:Wilson L (ed) Methods in cell biology 25, Academic Press, New York, pp 1–11
Weeds AG, Taylor RS (1975) Separation of subfragment-1 isoenzymes from rabbit skeletal muscle myosin. Nature 257: 54–56
Wohlfarth-Bottermann K-E (1974) Plasmalemma invaginations as characteristic constituents of plasmodia ofPhysarum polycephalum. J Cell Sci 16: 23–37
— (1975) Weitreichende fibrilläre Protoplasmadifferenzierungen und ihre Bedeutung für die Protoplasmaströmung. X. Die Anordnung der Actomyosinfibrillen in experimentell unbeeinflußten Protoplasmaadern vonPhysarum in situ. Protistologica 11: 19–30
— (1983) Dynamic cellular phenomena inPhysarum. Possibly accessible to laser techniques. In:Earnshaw JC, Steer MW (eds) The application of laser light scattering to the study of biological motion. Plenum Press, New York, London, pp 501–517 NATO ASI Series, Series A: Life Sciences 59
Wolf KV, Stockem W (1979) Studies on microplasmodia ofPhysarum polycephalum. III. Endocytotic activity, morphology of the vacuolar apparatus and defecation mechanism. Protoplasma 99: 125–138
-Hoffmann H-U,Stockem W (1979) Fine structure of the mucous layer and endocytotic activity of microplasmodia of the acellular slime moldPhysarum polycephalum. In:Sachsenmaier W (ed) Current research onPhysarum 120. Public Univ Innsbruck, pp: 187–190
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Brix, K., Kukulies, J. & Stockem, W. Studies on microplasmodia ofPhysarum polycephalum . Protoplasma 137, 156–167 (1987). https://doi.org/10.1007/BF01281151
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DOI: https://doi.org/10.1007/BF01281151