Summary
The microtubule cytoskeleton and cytoplasmic organization ofAllomyces macrogynus during zoosporogenesis was studied using light and electron microscopy. Indirect immunofluorescence methods revealed that the microtubule cytoskeleton progressed through three distinct stages of cytoplasmic distribution during zoospore development. During the first 10 minutes of zoosporogenesis, nuclei were strictly located in the periphery of the cytoplasm, and their associated centrosomes were positioned immediately adjacent to the plasma membrane. Microtubules emanated from centrosomes into the surrounding cytoplasm. Within 20 to 30 min after the induction of zoosporangial cleavage, nuclei migrated to new positions throughout the sporangial cytoplasm and microtubule arrays were primarily organized at and emanated from nuclear surfaces. During the final stage of zoosporogenesis, nuclear envelope-associated microtubules were not observed. Instead, primary organization of cytoplasmic microtubules returned to centrosomes (i.e., basal bodies) and flagella formation was evident. The MPM-2 antibody, which recognizes phosphorylated epitopes of several proteins associated with microtubule nucleation, stained centrosome regions throughout zoosporogenesis but did not stain nuclear envelopes.
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Abbreviations
- BSA:
-
bovine serum albumin
- DAPI:
-
4′,6-diamino-2-phenylindole
- dH2O:
-
deionized water
- DMSO:
-
dimethyl sulfoxide
- DS:
-
dilute salts solution
- G/5 0.1%:
-
glucose medium
- LN2 :
-
liquid nitrogen
- LSCM:
-
laser scanning confocal microscopy
- MTOC:
-
microtubule-organizing center
- PBS:
-
phosphate buffered saline
- PCM:
-
pericentriolar matrix
- TEM:
-
transmission electron microscopy
- VELM:
-
videoenhanced light microscopy
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Lowry, D.S., Roberson, R.W. Microtubule organization during zoosporogenesis inAllomyces macrogynus . Protoplasma 196, 45–54 (1997). https://doi.org/10.1007/BF01281057
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DOI: https://doi.org/10.1007/BF01281057