Summary
According to the hormonal conditions, after one month of culture shoots or somatic embryos could be obtained from leaf explants ofHelianthus smithii. Well-shaped embryos developed on media containing a combination of auxin and cytokinin, while on media containing only cytokinin shoots were observed. The primary leaves of these shoots resembled cotyledons. A detailed histological study of the regeneration process on three media, containing only cytokinin or auxin, or a combination of both, allowed the origin and development of the observed structures to be determined. All three conditions induced somatic embryos, which then developed differently and, within one month, finally gave rise to the two types of structures which were initially observed.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- MES:
-
2-(N-morpholino)ethane-sulfonic acid
- MS:
-
medium of Murashige and Skoog
- NAA:
-
1 -naphthaleneacetic acid
- TDZ:
-
thidiazuron
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Laparra, H., Bronner, R. & Hahne, G. Histological analysis of somatic embryogenesis induced in leaf explants ofHelianthus smithii Heiser. Protoplasma 196, 1–11 (1997). https://doi.org/10.1007/BF01281053
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DOI: https://doi.org/10.1007/BF01281053