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Nuclear DNA contents, rDNAs, chromatin organization, and karyotype evolution inVicia sect,faba

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Summary

Automated karyotype analyses, nuclear DNA contents, and sequences of rDNA internal transcribed spacers of the nine species inVicia sect.faba are reported. As karyomorphological parameters are used to evaluate the karyotype evolution, so the determination of the heterochromatin by Feulgen absorption at different thresholds of optical density provided further evidence on the chromatin organization withinVicia sect.faba. The comparison of sequences of rDNA spacers has enabled the definition of the phylogenetic relationships between the analyzed species.

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References

  • Alkin DJ, Goydir DJ, Bisby FA, While RJ (1986) Names and synonymous of species and subspecies of theViciae: issue 3.Viciae Database Project, Publication nr 7

  • Bain JF, Jansen RK (1995) A phylogenetic analysis of the aureoidSenecio (Asteraceae) complex based on ITS sequence data. Plant Syst Evol 195: 209–219

    Google Scholar 

  • Baldwin BG, Sanderson MJ, Porter JM, Woiciechowski MF, Campbell CS, Donoghue MJ (1995) The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann Missouri Bot Gard 82: 247–277

    Google Scholar 

  • Bennetzen JL, Kellogg EA (1997) Do plants have a one-way ticket to genomic obesity? Plant Cell 9: 1509–1514

    Google Scholar 

  • Ceccarelli M, Minelli S, Maggini F, Cionini PG (1995) Genome size variation inVicia faba. Heredity 74: 180–175

    Google Scholar 

  • Chooi WY (1971) Variation in nuclear DNA content in the genusVicia. Genetics 68: 195–211

    Google Scholar 

  • Cremonini R, Funari S, Mazzuca S (1992) Cytology ofVicia species: nuclear structure, karyological analysis and DNA content. Chromatin 1: 135–146

    Google Scholar 

  • —, Colonna N, Stefani A, Galasso I, Pignone D (1994) Nuclear DNA content, chromatin organization and chromosome banding in brown and yellow seeds ofDasypyrum villosum (L.) P. Candargy. Heredity 72: 365–373

    Google Scholar 

  • —, Miotto D, Api Ngu M, Tota D, Pignone D, Blangiforti S, Venora G (1998a) Cytology ofVicia species 5: nuclear chromatin structure, karyomorphological analysis and DNA content in newly discovered relatives ofVicia faba L. —Vicia kalakhensis Khattab., Maxted & Bisby andVicia eristalioides Maxted. Cytologia 63: 371–379

    Google Scholar 

  • —, Ruffini Castiglione M, Venora G, Blangiforti S, Losavio FP, Pignone D (1998b) Cytology ofVicia species 6: nuclear chromatin organization, karyomorphological analysis and DNA amount inVicia serratifolia Jacq. Caryologia 51: 195–205

    Google Scholar 

  • Felsenstein J (1993) PHYLIP (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle, Wash

    Google Scholar 

  • Frediani M, Cremonini R, Sassoli O (1992) Nuclear DNA characterisation of two species ofVicia: V. bithynica L. andV. narbonensis L. Biol Plant 34: 335–344

    Google Scholar 

  • Fuchs J, Pich U, Meister A, Schubert I (1994) Differentiation of field bean heterochromatin by in situ hybridization with a repeated FokI sequence. Chrom Res 2: 25–28

    Google Scholar 

  • Greilhuber J, Speta F (1976) C-banded karyotypes in theScilla hohenackeri group:S. persica andPuschkinia (Liliaceae). Plant Syst Evol 126: 149–188

    Google Scholar 

  • Hanelt P, Mettin D (1989) Biosystematics of the genusVicia L. Annu Rev Ecol Syst 20: 199–223

    Google Scholar 

  • Huziwara Y (1962) Karyotype analysis in some genera of Compositae VIII: further studies on the chromosomes ofAster. Am J Bot 49: 116–119

    Google Scholar 

  • Khattab AMA, Maxted N, Bisby FA (1988) Close relatives of the Faba bean from Syria: a new species ofVicia and notes onV. hyaeniscyamus (Leguminosae). Kew Bull 43: 535–540

    Google Scholar 

  • Kupicha FK (1976) Hie infrageneric structure ofVicia. Not R Bot Gard Edinb 34: 287–326

    Google Scholar 

  • Levan A, Freda K, Sandberg AA (1964) Nomenclature for centromeric position chromosomes. Hereditas 52: 201–220

    Google Scholar 

  • Maggini F, Barsanti P, Marazia T (1978) Individual variation of the NOR inAllium cepa andA. sativum. Chromosoma 66: 173–183

    Google Scholar 

  • —, Cremonini R, Zolfino C, Tucci GC, Delre V, De Pace C, Scarascia Mugnozza GT, Cionini PG (1991) Cytological localization of repeated sequences from the intergenic spacer of ribosomal DNA inVicia faba. Chromosoma 100: 229–234

    Google Scholar 

  • —, D'Ovidio R, Gelati MT, Frediani M, Cremonini R, Ceccarelli M, Minelli S, Cionini PG (1995) The FokI DNA repeats in the genome ofVicia faba: species-specificity, structure, redundancy modulation, nuclear organization. Genome 38: 1255–1261

    Google Scholar 

  • Maxted N (1988) A newVicia from Southwest Turkey. Not R Bot Gard Edinb 45: 453–456

    Google Scholar 

  • —, Khattab AMA, Bisby FA (1991) The newly discovered relatives ofVicia faba L. do little to resolve the enigma of its origin. Bot Chron 10: 435–465

    Google Scholar 

  • Pavone P, Salmeri C, Scammacca B (1995) “Karyo 95” nuovo programma di gestione dei dati cariologici. G Bot Ital 129: 2

    Google Scholar 

  • Perrino P, Pignone D (1981) Contribution to the taxonomy ofVicia species belonging to the sectionFaba. Kulturpflanze 29: 311–319

    Google Scholar 

  • Przybylska J, Zimniak-Przybylska Z (1995) Electrophoretic seed albumin patterns and species relationships inVicia sect.Faba. Plant Syst Evol 198: 179–194

    Google Scholar 

  • — —, Krajewski P (1998) Isoenzyme variation in the wild relatives ofVicia faba (Fabaceae). Plant Syst Evol 213: 173–186

    Google Scholar 

  • Raina SN, Bisht MS (1988) DNA amount and chromatin compactness inVicia. Genetica 77: 67–77

    Google Scholar 

  • —, Ogihara Y (1995) Ribosomal DNA repeat unit polymorphism in 49Vicia species. Theor Appl Genet 90: 477–486

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467

    Google Scholar 

  • Scott AJ, Knott M (1974) A cluster method for grouping means in the analysis of variance. Biometrics 30: 505–512

    Google Scholar 

  • Stebbins L (1971) Chromosomal evolution in higher plants. Edward Arnold, London

    Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680

    Google Scholar 

  • Venora G, Conicella C, Errico A, Saccardo F (1991) Karyotyping in plant by an image analysis system. J Genet Breed 45: 233–240

    Google Scholar 

  • —, Ocampo B, Singh KB, Saccardo F (1995) Karyotype of the Kabulitype chickpea (Cicer arietinum L.) by image analysis system. Caryologia 48: 147–155

    Google Scholar 

  • —, Blangiforti S, Cremonini R (1998) Karyotype analysis of twelve species belonging to genusVigna. Cytologia 64: 117–127

    Google Scholar 

  • Yakura K, Kato A, Tanifuji S (1987) Cytological localization of highly repeated DNA sequences, theFokI sequences family andBamHI sequences families, inVicia faba chromosomes. Jpn J Genet 62: 325–332

    Google Scholar 

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Authors and Affiliations

  1. Stazione Sperimentale di Granicoltura per la Sicilia, Caltagirone

    G. Venora & S. Blangiforti

  2. Dipartimento di Agrobiologia ed Agrochimica, Università della Tuscia, Viterbo

    M. Frediani, F. Maggini & M. T. Gelati

  3. Dipartimento di Scienze Botaniche, Università di Pisa, Via L. Ghini 5, I-56126, Pisa, Italy

    M. Ruffini Castiglione & R. Cremonini

Authors
  1. G. Venora
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  2. S. Blangiforti
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  3. M. Frediani
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  4. F. Maggini
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  5. M. T. Gelati
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  6. M. Ruffini Castiglione
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  7. R. Cremonini
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Correspondence to R. Cremonini.

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Venora, G., Blangiforti, S., Frediani, M. et al. Nuclear DNA contents, rDNAs, chromatin organization, and karyotype evolution inVicia sect,faba . Protoplasma 213, 118–125 (2000). https://doi.org/10.1007/BF01280512

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  • DOI: https://doi.org/10.1007/BF01280512

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