Summary
TherolD gene fromAgrobacterium rhizogenes has recently been shown to induce striking precocity of flowering in transgenic tobacco. A transcriptional fusion between 578 bp of therolD upstream regulating sequence and the GUS reporter gene has been transferred to tobacco plants and its expression analysed throughout the whole life cycle of the plant. A detailed histological analysis revealed thatrolD is strongly expressed in elongating and differentiating tissues of each organ, from the mature embryo to the adult plant. In organs with determinate growth, such as cotyledons, leaves and floral parts,rolD expression follows the age gradient of the tissues. In mature tissues,rolD expression is high, begins to decrease with ageing and is switched off when senescence occurs.RolD is also active in the vascular system, in the procambium, pith- and receptacular-meristems. Our data show that expression ofrolD is under developmental control as it correlates with, and may represent a molecular marker for, the elongation/expansion and maturation phases of the plant tissues.
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Abbreviations
- PCR:
-
polymerase chain reaction
- GUS:
-
β-glucuronidase
- X-Gluc:
-
5-bromo-4-chloro-3-indolyl-β-D-glucuronide
- SE:
-
standard error
- NT:
-
transcriptional terminator of the Nopaline synthase gene
- NOS-pro:
-
promoter of the Nopaline synthase gene
- NPT II:
-
Neomicine phosphotransferase II gene
- LB:
-
left border
- RB:
-
right border
- LS:
-
longitudinal sections
- TS:
-
transversal sections
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Trovato, M., Mauro, M.L., Costantino, P. et al. TherolD gene fromAgrobacterium rhizogenes is developmentally regulated in transgenic tobacco. Protoplasma 197, 111–120 (1997). https://doi.org/10.1007/BF01279889
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DOI: https://doi.org/10.1007/BF01279889