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A technique for optical and electron microscopy of isolated plant chromosomes

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Summary

Using a formalin fixation technique whole chromosomes from root tips ofVicia species were isolated into a buffer solution. Combining this technique with the use of a set of reciprocal translocations available for this species—Vicia faba— allowed each member of the karyotype to be isolated and identified.

Such isolated chromosomes can be used for optical microscopy or for either transmission or scanning electron microscopy where critical point drying clearly reveals chromatin fibre.

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Authors and Affiliations

  1. Department of Biological Sciences, Wye College, University of London, TN25 5AH, Ashford, Kent, UK

    G. P. Chapman & S. A. Cooke

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  1. G. P. Chapman
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  2. S. A. Cooke
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Recipient A.R.C. Grant Nr. AG 32/12.

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Chapman, G.P., Cooke, S.A. A technique for optical and electron microscopy of isolated plant chromosomes. Protoplasma 116, 198–200 (1983). https://doi.org/10.1007/BF01279838

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  • DOI: https://doi.org/10.1007/BF01279838

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