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Plant Chromosome Preparations and Staining for Light Microscopic Studies

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Plant Microtechniques and Protocols

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Abstract

Chromosome preparation is a major tool in cytogenetic analysis of an organism. Chromosome changes that occur in plant populations provide us with the evolutionary history of a species. Light microscopic observations of somatic cells provide basic information regarding the number and morphological characteristics of the chromosomes of a species. Investigations of meiotic chromosomes provide information regarding the structural changes and the way in which the chromosomes and genes segregate during sexual reproduction. Over the years several staining techniques have been developed by various researchers. In this chapter, we provide some selected methods of investigation of chromosomes for light microscopy.

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Author information

Authors and Affiliations

  1. Department of Applied Genetics, Karnataka University, 580003, Karnataka, Dharwad, India

    Subhash C. Hiremath

  2. Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada

    C. C. Chinnappa

Authors
  1. Subhash C. Hiremath
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  2. C. C. Chinnappa
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Corresponding author

Correspondence to Subhash C. Hiremath .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, University of Calgary, CALGARY, Alberta, Canada

    Edward Chee Tak Yeung

  2. Department of Plant Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

    Claudio Stasolla

  3. Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

    Michael John Sumner

  4. Center for Basic Research in Digestive Disease, Mayo Clinic , Rochester, Minnesota, USA

    Bing Quan Huang

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Hiremath, S., Chinnappa, C. (2015). Plant Chromosome Preparations and Staining for Light Microscopic Studies. In: Yeung, E., Stasolla, C., Sumner, M., Huang, B. (eds) Plant Microtechniques and Protocols. Springer, Cham. https://doi.org/10.1007/978-3-319-19944-3_16

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