Summary
Cytochrome oxidase (EC 1.9.3.1) activity of conidia of wild-typeNeurospora crassa was detected cytochemically at the ultrastructural level by 3,′-diaminobenzidine (DAB) reaction during heat treatment at 46 °C and after shift down to 25 °C. At 46 °C the decrease in the enzyme activity was shown to be time-dependent (0–10 hours) in the progressively overswelling conidia. Gradual recovery of the DAB reaction occurred in mitochondria of conidia outgrowing conidiogenous germ tubes (microcycle conidiation) when shifted down to 25 °C. Inactivation of cytochrome oxidase was efficiently prevented during heat treatment in the presence of Tween 20. Such conidia no longer overswelled but still required shift down to 25 °C to produce germ tubes with delayed conidiogenous ability.
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Ton-That, T.C., Michea-Hamzehpour, M. & Turian, G. Ultrastructural demonstration of loss and recovery of cytochrome oxidase activity during and after heat induction of microcycle conidiation inNeurospora crassa . Protoplasma 116, 149–154 (1983). https://doi.org/10.1007/BF01279832
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DOI: https://doi.org/10.1007/BF01279832