Summary
CulturedXenopus cells have been induced to fuse with carrot suspension cell protoplasts using PEG at high pH in the presence of high Ca2+. Ultrastructural observations confirm unambiguously that the fusion bodies seen by light microscopy are animal/plant cell heterokaryons. The csytoplasmic events occurring in theseXenopus/carrot fusion products during the first 48 hours of culture provide evidence for their viability. Some of the factors influencing the formation and subsequent survivalin vitro of interkingdom heterokaryons are discussed.
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Davey, M.R., Clothier, R.H., Balls, M. et al. An ultrastructural study of the fusion of cultured amphibian cells with higher plant protoplasts. Protoplasma 96, 157–172 (1978). https://doi.org/10.1007/BF01279583
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DOI: https://doi.org/10.1007/BF01279583