Summary
Synchronously dividing nuclei of the antheridial filaments ofChara vulgaris at the 32-celled stage have different structure depending on the period of interphase.
During S phase which begins as early as at the start of telophase (coincidently with the nuclear envelope formation and chromosome decondensation) one can observe a gradual reduction in the content of condensed chromatin, having the appearance of an indistinct network. During the middle S period the area of condensed chromatin decreases to the lowest level of about 29% of nuclear profile and the nuclear envelope becomes folded. At the end of S phase the condensed chromatin forms a more distinct and thicker reticulum which covers an area of about 52%.
During the early G2 phase, the area occupied by the condensed chromatin was about 41% and it was found to assume the shape of large and iregular clusters localized mainly near the nucleoli. The reticulate form of chromatin, characteristic of the S period, disappears almost completely. During the next period of interphase the condensed chromatin disperses considerably and covers now 24% of the area. At the end of the G2 phase the condensed chromatin reappears and transforms into chromosomes. Then the condensed chromatin removes from the nuclear envelope.
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References
Bajer, A., Molè-Bajer, J., 1969: Formation of spindle fibers, kinetochore orientation, and behavior of the nuclear envelope during mitosis in endosperm. Chromosoma27, 448–484.
Barlow, P. W., 1976: The relationship of the dispersion phase of chromocentric nuclei in the mitotic cycle to DNA synthesis. Protoplasma90, 381–392.
—, 1977: Changes in chromatin structure during the mitotic cycle. Protoplasma91, 207–211.
Comings, D. E., Okada, T. A., 1970: Condensation of chromosomes into the nuclear membrane during prophase. Exp. Cell Res.63, 471–473.
Erlandson, R. A., Harven, E., 1971: The ultrastructure of synchronized HeLa cells. J. Cell Sci.8, 353–397.
Forsberg, C., 1965: Nutritional studies ofChara in axenic cultures. Physiol. Plant.18, 275–289.
Frayssinet, C.,Bertaux, O.,Valencia, R., 1975: Aspects structuraux du noyau et du nucléole chezEuglena gracilis Z. au cours du cycle cellulaire. Colloques intern. du C.N.R.S. — Les cycles cellulaires et leur blocage chez plusieurs protistets. Gif-sur-Yvette.
Godlewski, M., 1977: The role of plant growth substances in the regulation of the cell cycle in antheridial filaments ofChara vulgaris L. I. Effect of gibberellic acid on some processes in the course of the cell cycle. Acta Soc. Bot. Pol.46, 317–329.
Gonzàlez-Fernàndez, G., Giménez-Martìn, G., Fernandez-Gómez, M. E., Torre, C. de la, 1974: Protein synthesis requirements at specific points in the interphase of meristematic cells. Exp. Cell Res.88, 163–170.
Heitz, D. H., 1929: Heterochromatin, Chromocentren, Chromomeren. Ber. dtsch. bot. Ges.47, 276–284.
Karling, J. S., 1926: Nuclear and cell division inNitella andChara. Bull. Tor. Bot. Club53, 229–318.
Klueva, T. S., Onishchenko, G. E., Poliakov, V. Yu., Chentsov, Yu. S., 1974: Ultrastruktura interfaznych klietok niekotorych rastienij na raznych stadiach klietochnogo cikla. Citologija14, 1465–1469.
Kuroiwa, T., 1973: Fine structure of interphase nuclei. VI. Initiation and replication sites of RNA synthesis in the nuclei ofPhysarum polycephalum as revealed by electron microscopic autoradiography. Chromosoma44, 291–299.
—,Tanaka, N., 1971: Fine structure of interphase nuclei. I. The morphological classification in interphase ofCrepis capillaris. Cytologia36, 143–160.
Kwiatkowska, M., Maszewski, J., 1976: Plasmodesmata between synchronously and asynchronously developing cells of the antheridial filaments ofChara vulgaris L. Protoplasma87, 317–327.
Lafontaine, J. G., Lord, A., 1974: An ultrastructural study and radioautographic study of evolution of the interphase nucleus in plant meristematic cells (Allium porrum). J. Cell Sci.14, 263–287.
Lima de Faria, A., Jaworska, H., 1968: Late DNA synthesis in heterochromatin. Nature217, 138–141.
Lord, A., Lafontaine, J. G., 1976: An ultrastructural and radioautographic study of the chromocentric interphase nucleus in plant meristematic cells (Raphanus sativus). J. Cell Sci.21, 193–207.
Matsui, S. I., Yosida, H., Weinfeld, H., Sandberg, A. A., 1972: Induction of prophase in interphase nuclei by fusion with metaphase cells. J. Cell Biol.54, 120–132.
Nagl, W., 1977: Early and late DNA replication in respectively condensed and decondensed heterochromatin ofAllium carinatum. Protoplasma91, 389–407.
Olszewska, M. J., 1974 a: The binding of3H actinomycin D in synchronously dividing cells of antheridial filaments and during spermatids differentiation ofChara vulgaris L. Bull. Acad. Pol. Sci. Sér. Biol. Cl. II22, 347–350.
—, 1974 b: Antheridial filaments ofChara vulgaris—a model for study of mitotic cycle and cell differentiation. Portug. Acta Biol. Sér. A.14, 313–322.
—, 1978: Chromatin condensation in late G2 phase as a factor related to the earlier initiation of mitosis in successive generations of antheridial filaments ofChara vulgaris L. Microscopica Acta80, 115–126.
—,Godlewski, M., 1972: An autoradiographic study of the synthesis of nucleic acids and protein during the cell cycle of synchronously dividing antheridial filaments inChara vulgaris L. Folia Histochem. Cytochem.10, 245–256.
—,Marciniak, K., 1977: The role of histones in the restriction of chromatin activity in successive stages of development of the antheridial filaments ofChara vulgaris L. Folia Histochem. Cytochem.15, 109–119.
Pickett-Heaps, J. D., 1969: Preprophase microtubule bands in some abnormal mitotic cells of wheat. J. Cell. Sci.4, 397–420.
—,Northcote, D. H., 1966: Organization of microtubules and endoplasmic reticulum during mitosis and cytokinesis in wheat meristem. J. Cell Sci.1, 109–120.
Robbins, E., Gonatas, N. K., 1964: The ultrastructure of a mammalian cell during the mitotic cycle. J. Cell Biol.21, 429–463.
Schwartzman, J. S., Díez, J. L., 1977: Late replicating DNA and the cell cycle ofAllium cepa. Cytobiologie14, 310–318.
Stack, S. M., Brown, D. B., Dewey, W. C., 1977: Visualization of interphase chromosomes. J. Cell Sci.26, 281–299.
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This work was supported by the Polish Academy of Sciences within the project 09.7.3.1.4.
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Kwiatkowska, M., Maszewski, J. Ultrastructure of the nuclei during different phases of the cell cycle in the antheridial filaments ofChara vulgaris L.. Protoplasma 96, 59–74 (1978). https://doi.org/10.1007/BF01279575
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DOI: https://doi.org/10.1007/BF01279575