Summary
The two tobacco cell lines TX 1 (wild type) and TX 4 (Palmer andWidholm 1975), which are respectively sensitive and resistant to pfluorophenylalanine, were studied by light and electron microscopy and scanning cytophotometry. The two cell lines differ in the following respects: TX 1 cells exhibit spherical cells, a chromatin organization like in free-grown plants ofNicotiana tabacum, and mainly diploid and haploid DNA values. TX 4 cells show large elongated (thread-like) cells, a diffuse nuclear ultrastructure, and mainly tetraploid and octoploid DNA values. Chromosome number counts indicate that dividing TX 1 cells own a karyotype close to the diploid one, while dividing TX 4 cells are around the tetraploid state. In addition, the effects of an exogenous polyamine, spermidine, on chromatin organization were investigated. While the wild type cell line, TX1, did not respond to the treatment, the mutant, fluorophenylalanine resistant cell line, TX4, exhibited reduced nuclear size and increased chromatin condensation.
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Nagl, W., Ribicki, R., Mertler, H.O. et al. The fluorophenylalanine sensitive and resistant tobacco cell lines, TX1 and TX 4 1. DNA contents, chromosome numbers, nuclear ultrastructures, and effects of spermidine. Protoplasma 122, 138–144 (1984). https://doi.org/10.1007/BF01279447
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DOI: https://doi.org/10.1007/BF01279447