Summary
A range of drugs was applied to developing pea seed cotyledons in an attempt to perturb the intracellular transport of newly synthesized vicilin through the endoplasmic reticulum and Golgi vesicles to its site of storage, the vacuole. The most pronounced effects, produced by the ionophores monensin and nigericin, were on Golgi-mediated transport. Unlike the situation in most other tissues that have been studied the number of Golgi vesicles did not increase, suggesting that their movement is not slowed or stopped. However, the Golgi-mediated transport of vicilin was redirected from the vacuole tonoplast to the plasmalemma and the newly synthesized vicilin was released from the cotyledon cells to accumulate between the plasmalemma and the cell wall.
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Craig, S., Goodchild, D.J. Golgi-mediated vicilin accumulation in pea cotyledon cells is re-directed by monensin and nigericin. Protoplasma 122, 91–97 (1984). https://doi.org/10.1007/BF01279441
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DOI: https://doi.org/10.1007/BF01279441