Summary
sensitive immunocytochemical method for the identification of laticifers has been developed. Frozen sections of various laticifer-bearing plant material, mounted on slides, were first flooded with the IgG fraction of rabbit anti-latex antiserum, prepared using whole latex ofAsclepias syriaca, then flooded with fluorescein-conjugated IgG fraction goat anti-rabbit IgG to visualize laticifers. Positive fluorescence was observed for laticifers in shoots and embryos ofA. syriaca andStapelia bella and embryos ofA. tuberosa. Laticifers did not fluoresce in shoots ofA. tuberosa andEuphorbia tirucalli, in embryos ofE. marginata, or in petioles ofMusa paradisiaca andCichorium intybus. Controls prepared with uninjected rabbit serum were negative (no fluorescence).
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References
Berlyn, G. P., Mikshe, J. P., 1976: Botanical Microtechnique and Cytochemistry, 1st ed., Ames, Iowa: The Iowa State University Press.
Biesboer, D. D., 1983: The detection of cells with a laticifer-like metabolism inAsclepias syriaca L. suspension cultures. Plant Cell Rep.2, 137–139.
Bruni, A., Dall'Olio, G., Mares, D., 1977: Use of fluorescent labeling methods in morphological and histochemical studies of latexin situ. Caryologia30, 486–487.
Bruni, A., Tosi, B., 1980: A method for localizing embryonal laticifers by combined conventional and fluorescent microscopy. Protoplasma102, 343–347.
Dall'Olio, G., Tosi, B., Bruni, A., 1978: A new rapid fluorescent labelling method for the detection of the latex tissues inEuphorbiaceae plants. Planta Med.34, 183–187.
Esau, K., 1965: Plant Anatomy, 2nd ed. New York: Wiley.
Metcalfe, C. R., 1967: Distribution of latex in the plant kingdom. Econ. Bot.21, 115–127.
Murashige, T., Skoog, F., 1962: A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant.15, 473–497.
Sternberger, L. A., Hardy, P. H., Cuculis, J. J., Meyer, H., 1970: The unlabelled antibody-enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem.18, 315.
Wilson, K. J., Nessler, C. L., Mahlberg, P. G., 1976: Pectinase inAsclepias latex and its possible role in laticifer growth and development. Amer. J. Bot.63, 1140–1144.
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Wilson, K.J., Petersen, B.H. & Biesboer, D.D. Immunocytochemical identification of laticifers. Protoplasma 122, 86–90 (1984). https://doi.org/10.1007/BF01279440
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DOI: https://doi.org/10.1007/BF01279440