Summary
Spindle membrane in the second division of meiosis inIris comprises endoplasmic reticulum (ER) and small spherical vesicles (40–80 nm diameter). The vesicles are in all respects similar to those of dictyosomes and it is proposed that they originate in the Golgi system. ER on the other hand is generated from the nuclear envelope (NE). Invaginations and evaginations of the inner and outer membranes of the NE are present at interphase and they enlarge during prophase II and break to form spindle ER elements at the beginning of prometaphase II. The area enclosed by this ER continues to increase until the entire spindle is filled with irregular profiles at mid-prometaphase II. During late-prometaphase II the quantity of ER decreases and, concurrent with an increase in the number of microtubules (MTs), the cisternae align alongside MT bundles and accumulate at the poles. It is suggested that ER increases in quantity by growth of NE membranes. Furthermore, the alignment of ER along the interpolar axis and its transport polewardsprior to anaphase suggest the action of a force on each ER element.
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References
Bajer, A., 1968: Behaviour and fine structure of spindle fibres during mitosis in endosperm. Chromosoma25, 249–281.
Esau, K., Gill, R. H., 1969: Structural relations between nucleus and cytoplasm during mitosis inNicotiana tabacum mesophyll. Can. J. Bot.47, 581–591.
Forer, A., Zimmermann, A., 1982: Cellular biodynamics: mitosis and cytokinesis. New York: Academic Press.
Harris, P., 1975: The role of membranes in the organisation of the mitotic apparatus. Exp. Cell Res.94, 409–425.
Hepler, P. K., 1977: Membranes in the spindle apparatus: their possible role in the control of microtubule assembly. In: Mechanisms and control of cell division (Rost, T. L., Gifford, E. M., eds.), pp. 212–232. Stroudsburg: Dowden, Hutchinson and Ross, Inc.
Hepler, P. K., 1980: Membranes in the mitotic apparatus of barley cells. J. Cell Biol.86, 490–499.
—,Wick, S. M., Wolniak, S. M., 1981: The structure and role of membranes in the mitotic apparatus. In: International cell biology 1980–1981 (Schweiger, H. G., ed.), pp. 673–686. Berlin-Heidelberg-New York: Springer.
-Wolniak, S. M., 1983: Membranes in the mitotic apparatus: their structure and function. Int. Rev. Cytol., in press.
Jackson, W. T., Doyle, B. G., 1982: Membrane distribution in dividing endosperm cells ofHaemantus. J. Cell Biol.94, 637–643.
Knobler, R. L., Stempak, J. G., Laurencin, M., 1978: Preparation and analysis of serial sections in electron microscopy. In: Principles and techniques of electron microscopy biological applications (Hayat, M. A., ed.), Vol. 8, pp. 113–155. New York: Van Nostrand Reinhold.
Lambert, A.-M., 1980: The role of chromosomes in anaphase trigger and nuclear envelope activity in spindle formation. Chromosoma76, 295–308.
Moens, P. B., Moens, T., 1981: Computer measurements and graphics of three dimensional cellular ultrastructure. J. Ultrastruct. Res.75, 131–141.
Moll, E., Paweletz, N., 1980: Membranes of the mitotic apparatus of mammalian cells. Eur. J. Cell Biol.21, 280–287.
Nicklas, R. B., 1971: Mitosis. Adv. Cell Biol.2, 225–294.
Paweletz, N., 1974: Elektronenmikroskopische Untersuchungen an frühen Stadien der Mitose bei Heia-Zellen. Cytobiologie9, 368–390.
—, 1981: Membranes in the mitotic apparatus. Cell Biol. Int. Rep.5, 323–336.
—,Finze, E. M., 1981: Membranes and microtubules of the mitotic apparatus of mammalian cells. J. Ultrastruct. Res.76, 127–133.
Roth, L. E., Wilson, H. J., Chakraborty, J., 1966: Anaphase structure in mitotic spindles typified by spindle elongation. J. Ultrastruct. Res.14, 460–483.
Ryan, K. G., 1980: The culture and correlative electron microscopy of pollen mother cells in meiosis: development of techniques and some observations on selected topics. Ph.D. thesis. Victoria University of Wellington, Wellington, New Zealand.
—, 1983 a: Chromosome movements in pollen mother cells: techniques for living cell ciné-microscopy and for electron microscopy. Mikroskopie40, 67–78.
—, 1983 b: Prometaphase and anaphase chromosome movements in living pollen mother cells. Protoplasma116, 24–33.
Schatten, G., Schatten, H., Simerly, C., 1982: Detection of sequestered calcium during mitosis in mammalian cell cultures and in mitotic apparatus isolated from sea urchin zygotes. Cell Biol. Int. Rep.6, 717–731.
Spiegel, F. W., 1982: Mitosis in the protostelidPlanoprotostelium aurantium. Protoplasma113, 178–188.
Taura, M., 1978: Origin and fate of paired cisternae in mitotic aortic cells of swine. J. Electron Microsc.27, 283–291.
Wilson, H. J., 1968: The fine structure of the kinetochore in meiotic cells ofTradescantia. Planta78, 379–385.
Wise, D., 1982: The ultrastructure of an intraspindle membrane system in meiosis of spider spermatocytes. J. Cell Biol.95 (2/2), 302 a.
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Ryan, K.G. Membranes in the spindle of Iris pollen mother cells during the second division of meiosis. Protoplasma 122, 56–67 (1984). https://doi.org/10.1007/BF01279437
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DOI: https://doi.org/10.1007/BF01279437