Summary
The osmium-ligand binding technique and scanning electron microscopy have been applied to the study of the three-dimensional organization of mesocarp cells of a mature avocado fruit. Using this approach the mitochondria of the cells appear as elongated, branching structures and the endoplasmic reticulum consists of a complex of tubular strands, vesiculated strands and lamellar sheets. Associations of the endoplasmic reticulum with other organelles are also apparent. It is suggested that this approach provides a valuable means to assess the structural transitions in cell organization that occur during development or with functional changes.
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Platt-Aloia, K.A., Thomson, W.W. Aspects of the three-dimensional intracellular organization of mesocarp cells as revealed by scanning electron microscopy. Protoplasma 104, 157–165 (1980). https://doi.org/10.1007/BF01279378
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DOI: https://doi.org/10.1007/BF01279378