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The involvement of F-actin inUromyces cell differentiation: The effects of cytochalasin E and phalloidin

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Summary

The role of F-actin in cell differentiation ofUromyces appendiculatus (bean rust fungus) germlings was examined by treating differentiating and nondifferentiating germlings with the actin-binding drugs cytochalasin E (CE) and phalloidin. Prolonged exposure of urediospores to 5×10−3–5 × 10−5 M CE induced nuclear division in up to 28–45% of the resulting germlings, whereas the rate of mitosis in established germlings exposed to these concentrations of CE was significantly lower (4–11%). Germlings treated with CE shifted from polarized apical growth to spherical expansion, cytoplasmic microfilaments were depolymerized, and nuclear inclusions became enlarged. Differentiating germlings exposed to a 10 minute pulse of 5×10−6M CE before the initiation of septum formation prevented the establishment of the F-actin septal ring and growth of the crosswall delimiting the appressorium. Although these CE treatments resulted in morphological and nuclear events similar to those occurring during normal appressorium formation, transient microfilament depolymerization was not sufficient to induce differentiation. Phalloidin stabilized cytoplasmic microfilaments, especially posteriorly-located microfilaments, but did not affect differentiation, nor did it significantly inhibit the effects of CE.

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Abbreviations

CE:

cytochalasin E

DAPI:

4,6-diamidino-2-phenylindole

DMSO:

dimethyl sulfoxide

F-actin:

filamentous actin

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Author notes

  1. B. E. Tucker

    Present address: Department of Botany and Microbiology, Arizona State University, 85287, Tempe, AZ, USA

Authors and Affiliations

  1. Department of Plant Pathology, New York State Agricultural Experiment Station, Cornell University, Geneva, NY

    B. E. Tucker & H. C. Hoch

  2. Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, N.Y.

    R. C. Staples

Authors
  1. B. E. Tucker
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  2. H. C. Hoch
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  3. R. C. Staples
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Tucker, B.E., Hoch, H.C. & Staples, R.C. The involvement of F-actin inUromyces cell differentiation: The effects of cytochalasin E and phalloidin. Protoplasma 135, 88–101 (1986). https://doi.org/10.1007/BF01277002

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  • DOI: https://doi.org/10.1007/BF01277002

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