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Inorganic cations inAllium cepa roots intracellular localizations

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Summary

The distribution of pyroantimonate-precipitable inorganic cation was studied inAllium cepa root tips using the direct potassium pyroantimonate fixation procedure. An accumulation of cation-antimonate precipitates was found at the boundary with the cell wall, presumably at the site of the plasmalemma; it extends to numerous infoldings at the cytoplasmic side suggesting a form of pinocytosis. A few large intramitochondrial antimonate deposits were also found and may reflect cation transport into mitochondria. The observed sites of inorganic cation accumulation in the nucleus apparently correlate with its RNA synthetic activity and reside mainly in the nucleolus.

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References

  • Bennett, H. S., 1969: The cell surface: movements and recombinations. In: Handbook of Molecular Cytology (A. Lima-de-Faria, ed.), pp. 1294–1317. Amsterdam-London: North Holland Publ. Co.

    Google Scholar 

  • Buvat, R., 1965: Le cytoplasme végétale. Masson ed. Paris.

    Google Scholar 

  • —, 1969: La cellule végétale. Hachette ed. Paris.

    Google Scholar 

  • —, 1969 b: Rôle des vacuoles dans les processus de renouvellement du cytoplasme. Port. Act. Biol. AXI, 347–358.

    Google Scholar 

  • Fisher, D. B., 1968: Localization of endogenous RNA polymerase activity in frozen sections of plant tissues. J. Cell Biol.39, 745–748.

    Google Scholar 

  • Hall, J. L., 1970: Pinocytotic vesicles and ion transport in plant cells. Nature (London)226, 1253–1254.

    Google Scholar 

  • Kierszenbaum, A. L., L. L. Tres, andC. J. Tandler, 1972: The distribution of inorganic cations during spermiogenesis in the mouse. J. Cell Biol.53, 239–243.

    Google Scholar 

  • Mayo, M. A., andE. C. Cocking, 1969: Detection of pinocytic activity using selective staining with phosphotungstic acid. Protoplasma68, 231–235.

    Google Scholar 

  • Mollenhauer, H. H., W. G. Whaley, andJ. H. Leech, 1961: A function of the Golgi apparatus in outer root cap cells. J. Ultrastruct. Res.5, 193–200.

    Google Scholar 

  • Northcote, D. H., J. D. Pickett-Heaps, 1966: A function of the Golgi apparatus in polysaccharide synthesis and transport in the root cap cells of wheat. Biochem. J.98, 159–167.

    Google Scholar 

  • Oki, K., S. Yoshioka, K. Hayashi, andM. Masuda, 1965: Mitochondrial changes induced by iron absorption in the duodenal absorptive cells of rats. J. Cell Biol.24, 328–332.

    Google Scholar 

  • Podolsky, R. J., T. Hall, andS. L. Hatchett, 1970: Identification of oxalate precipitates in striated muscle fiters. J. Cell Biol.44, 699–702.

    Google Scholar 

  • Poux, N., 1967: Localization d'activités enzymatiques dans les cellules du méristème radiculaire deCucumis sativus L. I. Activités phosphatasiques neutres. J. Microscopie6, 1043–1058.

    Google Scholar 

  • —, 1970: Localization d'activités enzymatiques dans le méristème radiculaire deCucumis sativus L. III. Activité phosphatasique acide. J. Microscopie9, 407–434.

    Google Scholar 

  • Reynolds, E. S., 1963: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–212.

    Google Scholar 

  • Roland, J. C., etB. Vian, 1971: Reactivité du plasmalemma végétal. Etude cytochimique. Protoplasma73, 121–137.

    Google Scholar 

  • Tandler, C. J., andA. L. Kierszenbaum, 1971: Inorganic cations in rat kidney. Localization with potassium pyroantimonate-perfusion fixation. J. Cell Biol.50, 830–839.

    Google Scholar 

  • —,C. M. Libanati, andC. A. Sanchis, 1970: The intracellular localization of inorganic cations with potassium pyroantimonate. Electron microscope and microprobe analysis. J. Cell Biol.45, 355–366.

    Google Scholar 

  • —,C. A. Sanchis, andA. J. Solari, 1969: Nucleolar orthosphosphate ions. Electron microscope and diffraction studies. J. Cell Biol.41, 91–108.

    Google Scholar 

  • Tjio, J. H., andA. Levan, 1951: The use of oxiquinoline in chromosome analysis. Ann. Estac. Exp. Aula Dei2, 21–64.

    Google Scholar 

  • Tres, L. L., A. L.Kierszenbaum, and C. J.Tandler, 1972: Inorganic cations in the cell nucleus. Selective accumulation during meiotic prophase in mouse testis. J. Cell Biol. (May).

  • Whaley, W. G., J. E. Kephart, andH. H. Mollenhauer, 1964: The dynamics of cytoplasmic membranes during development. In: Cellular membranes in development (M. Locke, ed.), pp. 135–173. New York and London: Academic Press.

    Google Scholar 

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Dr.Tandler is an established investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina.

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Tandler, C.J., Risueño, M.C. & Fernández-Gómez, M.E. Inorganic cations inAllium cepa roots intracellular localizations. Protoplasma 77, 201–210 (1973). https://doi.org/10.1007/BF01276758

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  • DOI: https://doi.org/10.1007/BF01276758

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