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Microfibril deposition on cultured protoplasts ofVicia hajastana

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Summary

Cell wall regeneration by protoplasts fromVicia hajastana suspension cultures was investigated with Calcofluor White ST staining and platinum-palladium surface replicas. Microfibril deposition was initiated after 10–20 minutes of culture and within 20 hours protoplasts were covered with a heavy mat of microfibrils. The early stages of microfibril formation could not be detected with Calcofluor staining.

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Author notes

  1. L. C. Fowke

    Present address: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

Authors and Affiliations

  1. Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    F. A. Williamson, G. Weber, F. Constabel & O. Gamborg

  2. Prairie Regional Laboratory, National Research Council, Saskatoon, Saskatchewan, Canada

    F. A. Williamson, L. C. Fowke, G. Weber, F. Constabel & O. Gamborg

Authors
  1. F. A. Williamson
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  2. L. C. Fowke
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  3. G. Weber
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  4. F. Constabel
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  5. O. Gamborg
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Additional information

Supported by the National Research Council of Canada, Grant A6304.

Supported by Deutsche Forschungsgemeinschaft.

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Williamson, F.A., Fowke, L.C., Weber, G. et al. Microfibril deposition on cultured protoplasts ofVicia hajastana . Protoplasma 91, 213–219 (1977). https://doi.org/10.1007/BF01276736

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  • DOI: https://doi.org/10.1007/BF01276736

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