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Isolation of organelles from carrot cell suspension cultures

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Summary

Organelles isolated from carrot cell suspension cultures by density gradient centrifugation and identified by their specific marker enzymes were found at the following mean densities on the sucrose gradient: microbodies 1.25 g/cm3 (catalase), mitochondria 1.18 (fumarase), endoplasmic reticulum 1.09 g/cm3 (NADH-cytochrome c reductase). Further enzyme assays were done for characterization of microbodies from carrot cultures.

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Author notes

  1. H. -D. Gregor

    Present address: Institut für Pflanzenphysiologie und Zellbiologie, Freie Universität Berlin, Berlin

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    1. H. -D. Gregor
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    This work was supported by the Deutsche Forschungsgemeinschaft.

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    Gregor, H.D. Isolation of organelles from carrot cell suspension cultures. Protoplasma 91, 201–205 (1977). https://doi.org/10.1007/BF01276734

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    • DOI: https://doi.org/10.1007/BF01276734

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