Summary
The examination of adjacent sections ofPolytoma papillatum cells by the electron microscope enabled us to construct three dimensional models of the chondriomes. These reconstructions show that in two predivision cells the chondriome primarily consists of one large and highly convoluted mitochondrion. One and three small ovoid mitochondria also exist. Examination of two new daughter cells reveales 30 and 47 mitochondria of various shapes and sizes. This indicates that during the growth phase ofPolytoma (vegetative cell cycle) preponderantly one mitochondrion is formed by fusion of those numerous mitochondria found after cell cleavage. Our observations will be compared with results on this subject in other organisms.
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References
Arnold, C. G., undO. Schimmer, 1970: Die Lokalisation extrakaryotischer Gene beiChlamydomonas reinhardii. Ber. dtsch. bot. Ges.83, 363–367.
— —,F. Schötz, undH. Bathelt, 1972: Die Mitochondrien vonChlamydomonas reinhardii. Arch. Mikrobiol.81, 50–67.
Atkinson, A. W., P. C. L. John, andB. E. S. Gunning, 1974: The growth and division of the single mitochondrion and other organelles during the cell cycle ofChlorella, studied by quantitative stereology and three dimensional reconstruction. Protoplasma81, 77–109.
Bromberg, R., 1974: Mitochondrial fragmentation during germination inBlastocladiella emersonii. Developmental Biol.36, 187–194.
Burton, M. D., andJ. Moore, 1974: The mitochondrion of the flagellate,Polytomella agilis. J. Ultrastruct. Res.48, 414–419.
Calvayrac, R., R. A. Butow, andM. Lefort-Tran, 1972: Cyclic replication of DNA and changes in mitochondrial morphology during the cell cycle ofEuglena gracilis (Z.). Exp. Cell Res.71, 422–432.
— — —, etR. Valencia, 1974: Généralisation du cycle mitochondrial chezEuglena gracilis Z. en cultures synchrones, hétérotrophe et phototrophe. Protoplasma80, 355–370.
Cavalier-Smith, T., 1974: Basal body and flagellar development during the vegetative cell cycle and the sexual cycle ofChlamydomonas reinhardii. J. Cell Sci.16, 529–556.
Grimes, C. W., H. R. Mahler, andP. S. Perlman, 1974: Nuclear gene dosage effect on mitochondrial mass and DNA. J. Cell Biol.61, 565–574.
Grobe, B., andC. G. Arnold, 1975: Evidence of a large, ramified mitochondrium inChlamydomonas reinhardii. Protoplasma86, 291–294.
Hoffmann, H.-P., andC. J. Avers, 1973: Mitochondrion of yeast: Ultrastructural evidence for one giant, branched organelle per cell. Science181, 749–751.
Johnson, U., andK. R. Porter, 1968: Fine structure of cell division inChlamydomonas reinhardii: basal bodies and microtubules. J. Cell Biol.38, 403–425.
Keddie, F. M., andKeddie, L., Barajas, J., 1969: Three-dimensional reconstruction ofPityrosporum yeast cells based on serial section electron microscopy. J. Ultrastruct. Res.29, 260–275.
Osafune, T., 1973: Three dimensional structures of giant mitochondria, dictyosomes and “concentric lamellar bodies” formed during the cell cycle ofEuglena gracilis (Z.) in synchronous culture. J. Electron Micr.22, 51–61.
—,S. Mihara, E. Hase, andI. Ohkuro, 1972 a: Electron microscope studies on the vegetative cellular live cycle ofChlamydomonas reinhardi Dangeard in synchronous culture. I. Some characteristics of changes in subcellular structures during cell cycle, especially in formation of giant mitochondria. Plant Cell Physiol.13, 211–227.
— — — —, 1972 b: Electron microscope studies of the vegetative cellular life cycle ofChlamydomonas reinhardi Dangeard in synchronous culture. II. Association of mitochondria and the chloroplast at an early developmental stage. Plant Cell Physiol.13, 981–989.
— — — —, 1975 a: Electron microscope studies of the vegetative cellular life cycle ofChlamydomonas reinhardi Dangeard in synchronous culture. III. Three-dimensional structures of mitochondria in the cells at intermediate stages of the growth phase of the cell cycle. J. Electron Micr.24, 247–252.
— — — —, 1975 b: Formation and division of giant mitochondria during the cell cycle ofEuglena gracilis Z. in synchronous culture. III. Three dimensional structure of mitochondria after division of giant forms. J. Electron Micr.24, 283–286.
— — — —, 1975 c: Formation and division of giant mitochondria during the cell cycle ofEuglena gracilis Z. in synchronous culture. I. Some characteristics of changes in the morphology of mitochondria and oxygen-uptake activity of cells. Plant Cell Physiol.16, 313–326.
Pellegrini, M., et L. Pellegrini, 1976: Continuité mitochondriale et discontinuité plastidale chezlÉuglena gracilis. Z. C. R. Acad. Sci. (Paris), Ser. D.282, 357–360.
Pratt, S. A., 1968: An electron microscope study of Nebenkern formation and differentiation in spermatids ofMurgantia histrionica (Hemiptera, Pentatomidae). J. Morph.126, 31–66.
Schimmer, O., undC. G. Arnold, 1970 a: Untersuchungen über Reversions- und Segregationsverhalten eines außerkaryotischen Gens vonChlamydomonas reinhardii zur Bestimmung des Erbträgers. Molec. gen. Genetics107, 281–290.
— —, 1970 b: Über die Zahl der Kopien eines außerkaryotischen Gens beiChlamydomonas reinhardii. Molec. gen. Genetics107, 366–371.
— —, 1970 c: Hin- und Rücksegregation eines außerkaryotischen Gens beiChlamydomonas reinhardii. Molec. gen. Genetics108, 33–40.
— —, 1970 d: Die Suppression der außerkaryotisch bedingten Streptomycin-Abhängigkeit beiChlamydomonas reinhardii. Arch. Microbiol.73, 195–200.
Schötz, F., 1972: Dreidimensionale, maßstabgetreue Rekonstruktion einer grünen Flagellatenzelle nach Elektronenmikroskopie von Serienschnitten. Planta (Berl.)102, 152–159.
Siu, C.-H., H. Swift, andK.-S. Chiang, 1976: Characterization of cytoplasmic and nuclear genomes in the colorless algaPolytoma. I. Ultrastructural analysis of organelles. J. Cell Biol.69, 352–370.
Spurr, A. R., 1969: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res.26, 31–43.
Triemer, R. E., andR. M. Brown Jr., 1974: Cell division inChlamydomonas moewusii. J. Phycol.10, 419–433.
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We are greatly indebted to Prof. Dr. C. G.Arnold (Erlangen) for his support in this work.
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Gaffal, K.P., Kreutzer, D. The mitochondria ofPolytoma papillatum at two different stages of the vegetative cell cycle. Protoplasma 91, 167–177 (1977). https://doi.org/10.1007/BF01276731
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DOI: https://doi.org/10.1007/BF01276731