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Über die Wirkung von ADP und einigen Kationen auf die Rotationsströmung in den Wurzelhaaren der Gerste (Hordeum vulgare L.)

The effect of ADP and some cations on rotational streaming in barley (Hordeum vulgare L.) root hairs

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Summary

The effect of ADP, Ca++, Mg++, K+, and Cu++ upon rotational streaming within barley (Hordeum vulgäre L.) root hairs was separately studied. It was shown that various solutions of ADP may stimulate the streaming after continuous treatment. The rate increase of the rotational streaming was inverse proportional to ADP concentration (Fig. 3).

From the investigated cations only Ca++ (1·10−3M) caused a stimulation of streaming after continuous treatment. This effect is probably due to enzymic activation of a contractile proteine which has ATPase feature.

The role of ADP and of the investigated cations in the stimulation of the rotational streaming was studied by means of mixed treatment. This kind of treatment consists in a simultaneous administration of ADP (1 · 10−6M) and CaCl2, MgCl2, KCl (1 · 10−3M), or CuCl2 (1 · 10−6M) solutions. Ca++ and Mg++ showed an antagonistic action. Ca++ brings about an immediately suppress of ADP induced stimulation. Suddenly the rate of streaming comes back to control. Mg++ after a temporary maintaining of stimulation, also causes the lowering of the streaming. The action of K+ was very similar to those of Ca++. Cu++ changes to a little extent the stimulation caused by ADP.

The simultaneous action of ADP and of the investigated cations allow us to express the following hypothesis. The stimulation of the rotational streaming after ADP treatment probably is due to ATP synthetized in mitochondria on the account of ADP. The additional synthesis of ATP can be prevented by simultaneous administration of Ca++. According toHanson and his coworkers Ca++ would compete with ADP for a phosphorylated intermediate product. From a such competition would result the Ca++ and Pi accumulation. The active uptake of salts which require energy would also explain the lowering of the rotational streaming rate after the mixed treatment.

Zusammenfassung

In getrennten Versuchen wurde die Wirkung von ADP, Ca++, Mg++, K+ und Cu++ auf die Rotationsströmung in den Wurzelhaaren der Gerste (Hordetim vulgare L.) untersucht. Das in verschiedenen Konzentrationen fortdauernd verabreichte ADP bedingte eine Stimulation der Plasmaströmung. Die Beschleunigung der Rotationsströmung war der ADP-Konzentration gegenüber umgekehrt proportional (Abb. 3).

Von den untersuchten Kationen hatte nur Ca++ (1·10−3 Mol) eine Stimulationswirkung. Diese Stimulationswirkung wird der Aktivierung eines Enzyms bzw. eines kontraktilen Proteins mit ATPase-Eigenschaften zugeschrieben.

Die Rolle von ADP und einigen Kationen bei der Stimulation der Rotation wurde dann mit Hilfe einer gemischten Behandlung untersucht. Diese bestand in der gleichzeitigen Verabreichung von ADP (1·10−6 Mol) und CaCl2, MgCl2, KCl (1 · 10−3 Mol) oder CuCl2 (1·10−6 Mol). Es wurde festgestellt, daß Mg++ und Ca++ eine antagonistische Wirkung ausüben. Ca++ hebt die durch ADP induzierte Stimulation auf und reduziert die Rotationsgeschwindigkeit plötzlich bis auf den Kontrollwert. Die Mg++-Wirkung bewirkt, nach einer zeitweiligen Beibehaltung der Stimulation, ebenfalls eine Abnahme der Geschwindigkeit. K+ hat eine ähnliche Wirkung wie Ca++. Cu++ beeinträchtigt die ADP-induzierte Stimulation in geringem Maße.

Die gleichzeitige Einwirkung von ADP und einigen Kationen erlaubt die Aufstellung folgender Hypothese. Die Rotationsstimulation erfolgt dank dem ATP, das auf Kosten des von außen absorbierten ADP in den Mitochondrien synthetisiert wird. Die zusätzliche ATP-Synthese kann durch gleichzeitige Ca++-Behandlung unterbunden werden. NachHanson und Mitarb, sollen Ca++ und ADP um ein phosphoryliertes Zwischenprodukt in Kompetition treten, so daß es zu einer Ansammlung von Ca++ und Pa in der Zelle kommt. Andererseits könnte teilweise auch die aktive, energieverbrauchende Salzabsorption die Geschwindigkeitsabnahme der Rotation bei gemischter Behandlung erklären.

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Authors and Affiliations

  1. Zweigstelle der rumänischen Akademie Cluj, Rumänien

    Georgeta Lazăr-Keul, Viorel Soran & Martin Keul

  2. Abteilung für Cytophysiologie der Pflanzen, Biologisches Forschungszentrum, Rumänien

    Georgeta Lazăr-Keul, Viorel Soran & Martin Keul

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  1. Georgeta Lazăr-Keul
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Lazăr-Keul, G., Soran, V. & Keul, M. Über die Wirkung von ADP und einigen Kationen auf die Rotationsströmung in den Wurzelhaaren der Gerste (Hordeum vulgare L.). Protoplasma 69, 37–48 (1970). https://doi.org/10.1007/BF01276650

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