Summary
Several cytochemical techniques and routine electron microscopy were used to detect changes in the pea cotyledonary bud nucleolus, from the G0–1 resting state until the first mitoses (24th hour) following removal of the main stem. In the G0–1 nucleus the fibrillar RNP nucleolus, shaped like a hollow sphere, possessed clumps of DNA connected to the compact peripheral nuclear chromatin by DNA fibres. The large central space, surrounded by the fibrillar component, showed the characteristics of a heterogeneous fibrillar center. By the 6th hour, this heterogeneous fibrillar center broke up, into small scattered elements in which DNA was detected. Between the 9th and 12th hours, RNA maturation produced a granular zone. Inter- and peri-chromatin granules of RNP were then present. In the bud, which was either in the resting state or in the process of reactivation, Ag-NOR proteins were located throughout the nucleolar mass, but were heavily concentrated in its peripheral region; they constituted a binding network between the various nucleolar components. The role and importance of the G0–1 nuclei karyosome, present until the 9th hour, is discussed.
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Jennane, A., Landré, P. & Nougarède, A. Nucleolus activation in pea cotyledonary buds during 24 hours after decapitation of the main stem: Cytochemical studies. Protoplasma 136, 29–36 (1987). https://doi.org/10.1007/BF01276315
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DOI: https://doi.org/10.1007/BF01276315