Summary
Electron microscopy of thin sections ofCrithidia fasciculata showed cytoplasmic organelles 0.2–0.8 μm in diameter containing a finely granular matrix limited by a single membrane. Utilization of cytochemical techniques (DAB) for endogenous catalase revealed pronounced deposition of a highly electron-opaque material in these organelles. Reaction of cells with DAB was completely inhibited by 0.02 M 3-amino-1,2,4-triazole. It is concluded that this organelle is a microbody on the basis of cytological and cytochemical similarities with microbodies from plant and other animal cells.
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This work was supported in part by research grant NIAID-USPHS AI09445.
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Muse, K.E., Roberts, J.F. Microbodies inCrithidia fasciculata . Protoplasma 78, 343–348 (1973). https://doi.org/10.1007/BF01275702
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DOI: https://doi.org/10.1007/BF01275702