Summary
Cell volume calculations are often used to estimate biomass of natural phytoplankton assemblages. Such estimates may be questioned due to morphological differences in the organisms present. Morphometric analysis of 8 species representative of phytoplankton types found in the Great Lakes shows significant differences in cell constituent volumes. Volume of physiologically inert wall material ranges from nil, in some flagellates, to over 20% of the total cell volume in certain diatoms. Likewise, “empty” vacuole may comprise more than 40% of the total cell volume of some diatoms, but less than 3% of the volume of some flagellates. In the organisms investigated, the total carbon containing cytoplasm ranged from 52% to 98% of the total cell volume and the metabolizing biovolume ranged from 30% to 82%. Although these differences complicate direct biomass estimation, morphometric analysis at the ultrastructural level may provide ecologically valuable insights.
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References
Atkinson, A. W. Jr., P. C. L. John, andB. E. S. Gunning, 1974: The growth and division of the single mitochondrion and other organelles during the cell cycle ofChlorella, studied by quantitative stereology and three dimensional reconstruction. Protoplasma81, 77–109.
Bellinger, E. G., 1974: A note on the use of algal sizes in estimates of population standing crops. Br. Phycol. J.9, 157–161.
Brown, T. E., andF. L. Richardson, 1968: The effect of growth environment on the physiology of algae: Light intensity. J. Phycol.4, 38–54.
Chalkey, H. W., 1943: Methods for the quantitative morphologic analysis of tissues. J. nat. Cancer Inst.4, 47.
Collyer, D. M., andG. E. Fogg, 1955: Studies on fat accumulation by algae. J. exp. Bot.6, 256–275.
Delesse, M. A., 1847: Procédé mécanique pour determiner la composition des roches. C. R. Acad. Sci. (Paris)25, 444.
Fogg, G. E., 1966: Algal cultures and Phytoplankton ecology, 126 pp. Madison, Wisconsin: University of Wisconsin Press.
Glagoleff, A. A., 1933: On the geometrical methods of quantitative mineralogic analysis of rocks. Tr. Inst. Econ. Min. and Metal, Moscow. Volume 59.
Hibberd, D. J., 1976: The ultrastructure of theChrysophyceae andPrymnesiophyceae (Haptophyceae): a survey with some new observations on the ultrastructure of theChrysophyceae. Bot. J. Lin. Soc.72, 55–80.
Holmes, R. W., 1966: Light microscope observations on cytological manifestations of nitrate, phosphate, and silicate deficiency in four marine centric diatoms. J. Phycol.2, 136–140.
Humphreys, R. E., 1973: Sucrose transport at the tonoplast. Phytochem.12, 1201–1219.
Laties, G. G., 1969: Dual mechanisms of salt uptake in relation to compartmentation and long distance transport. Ann. Rev. Plant Physiol.20, 89–116.
Lohmann, H., 1908: Untersuchungen zur Feststellung des vollständigen Gehaltes des Meeres an Plankton. Wiss. Meeresuntersuch. Abt. Kiel N. F.10, 131–370.
Loud, A. V., 1968: A quantitative stereological description of the ultrastructure of normal rat liver parenchymal cells. J. Cell Biol.37, 27–45.
Luft, J. H., 1961: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol.9, 409–414.
Messer, G., andY. Ben-Shaul, 1972: Changes in chloroplast structure during culture growth ofPeridinium cinctum Fa.Westii (Dinophyceae). Phycologia11, 291–299.
Mullin, M. M., P. R. Sloan, andP. W. Eppley, 1966: Relationship between carbon content, cell volume, and area in phytoplankton. Limnol. Oceanogr.11, 307–311.
Nalewajko, C., 1966: Dry weight, ash, and volume data for some freshwater planktonic algae. J. Fish. Res. Bd. Can.23, 1285–1288.
Paasche, E., 1960: On the relationship between primary production and standing stock of phytoplankton. J. Conseil, Conseil Perm. Intern. Exploration Mer.26, 33–48.
Reynolds, E. S., 1963: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol.17, 208–212.
Sorokin, C., andR. W. Krauss, 1958: The effect of light intensity on the growth rates of green algae. Plant Physiol.33, 109–113.
— —, 1965: The dependence of cell division inChlorella on temperature and light intensity. Amer. J. Bot.52, 331–339.
Stempak, J. F., andR. T. Ward, 1964: An improved staining method for electron microscopy. J. Cell Biol.22, 697–701.
Strathmann, R. R., 1967: Estimating the organic carbon content of phytoplankton from cell volume or plasma volume. Limnol. Oceanogr.12, 411–418.
Underwood, E. E., 1970: Quantitative Stereology, 274 pp. Reading, Mass.: Addison-Wesley.
Vollenweider, R. A., M. Munawar, andP. Stadelmann, 1974: A comparative review of phytoplankton and primary production in the Laurentian Great Lakes. J. Fish. Res. Bd. Can.31, 739–762.
Weibel, E. R., andR. B. Bolender, 1973: Stereological techniques for electron microscopic morphometry. In: Principles and techniques of electron microscopy. Biological Applications (Hayat, M. A., ed.), Volume 3, pp. 239–296. New York: Van Nostrand Reinhold.
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Sicko-Goad, L., Stoermer, E.F. & Ladewski, B.G. A morphometric method for correcting phytoplankton cell volume estimates. Protoplasma 93, 147–163 (1977). https://doi.org/10.1007/BF01275650
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DOI: https://doi.org/10.1007/BF01275650