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Ultrastructural changes of organelles in coleoptile cells during anaerobic germination of rice seeds

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Summary

Ultrastructural changes of organelles, especially those of mitochondria in rice seedlings germinated under strictly anaerobic conditions were investigated.

The embryos of dry seeds had slightly modified mitochondria, characterized by an electron transparent matrix with few cristae and electron opaque patches. These mitochondria developed normally for 48 hours irrespective of whether oxygen was present or not. However, after 72 hours' germination under anaerobic conditions vesiculation of the cristae developed and progressed greatly for the subsequent 24 hours and most of the spaces in the mitochondrion became occupied with vacuolated cristae. Vesiculation seemed to be the effect of cessation of energy supply from the mitochondria themselves.

Ultrastructural changes of other organelles such as the plastids and endoplasmic reticulum were also observed after 96 hours under anaerobic conditions.

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Author notes

  1. Katsumi Ueda

    Present address: Biological Laboratory, Women's University, Nara

  2. Hideo Tsuji

    Present address: Laboratory for Plant Ecological Studies, Faculty of Science, Kyoto University, Kyoto, Japan

Authors and Affiliations

  1. Biological Laboratory, Women's University, Nara

    Hideo Tsuji

  2. Laboratory for Plant Ecological Studies, Faculty of Science, Kyoto University, Kyoto, Japan

    Katsumi Ueda

Authors
  1. Katsumi Ueda
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  2. Hideo Tsuji
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Ueda, K., Tsuji, H. Ultrastructural changes of organelles in coleoptile cells during anaerobic germination of rice seeds. Protoplasma 73, 203–215 (1971). https://doi.org/10.1007/BF01275595

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  • DOI: https://doi.org/10.1007/BF01275595

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